Osmosis and Plant Cells.

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David Biagioni

Osmosis and Plant Cells.

In this experiment I intend to look at how osmosis affects plant cells.

The aim of this experiment is to investigate the movement of water into and out of plant cells by osmosis. The cells chosen for study will be potato strips. A definition of osmosis from 'Britannica Encyclopedia' is: "the flow of water by diffusion through a differentially permeable membrane from areas of high water concentration to regions of low water concentration." I plan to investigate this phenomenon using the methods expressed below:

My Procedure

For this experiment I plan to cut out eighteen thin strips of potatoes of uniform size. I shall measure these out using a vernier calliper and then cut them as accurately as I can, using a scalpel.

Now I shall put six test tubes into a test tube rack. I shall label these with sticky labels to show what solutions they shall contain. The solutions I shall use in strength of molars are: 0, 0.2, 0.4, 0.6, 0.8, and 1.0. I shall then measure out the solutions for each test tube using a burette and pied pump. Each test tube shall contain twenty ml of the selected solution.

Once all the test tubes are set up, three strips of potato shall be inserted into each of the six test tubes. On a stopwatch I will then time twenty minutes (1200 seconds) and after twenty minutes the potato strips will be taken out of the solution and measured using a vernier calliper. My results will then be recorded on a table, which I shall then work out the average percentage increase and decrease on.

Safety

I plan to conduct my experiment safely by cutting the potato strips carefully on a cutting board. The desk shall be cleared with only necessary equipment out. The test tube rack shall be kept in the middle of the desk to prevent it from falling onto the floor. Stools and bags shall be put away under the desk and incase of spillage, paper towels will be kept nearby to clean up with.
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Apparatus

This is my list of equipment I will need to conduct my experiment as my plan shows below;

* 1 Potato

* 1 scalpel

* 1 Cutting Board

* 1 vernier Calliper

* 1 Digital Stop Watch

* Graduated Pipette and Pi-Pump

* 6 Labels, one for each test tube

* 6 Glass Test Tubes, all of the same size

* 60ml of Distilled Water

* 60ml of 0.2 Molar Sucrose Solution

* 60ml of 0.4 Molar Sucrose Solution

* 60ml of 0.6 ...

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