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To investigate a certain factor that will affect the rate of breakdown of the protein gelatine using trypsin.

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Introduction

Aim: To investigate a certain factor that will affect the rate of breakdown of the protein gelatine using trypsin. Key factors: Trypsin is an enzyme. The speed that enzymes work can be affected by many factors such as volume, temperature and pH. My independent variable is temperature. This will affect how fast the trypsin works. I will measure and control this using a thermometer. My dependent variable is time. I will measure the difference in speeds of the enzyme at different temperatures (with a stopwatch) in seconds (rather than in minutes and seconds) so that it is easier to compare and put into graphs. My controlled variables will be: pH of enzyme solution (measured using universal indicator), Volume of trypsin solution (using a measuring cylinder) ...read more.

Middle

Basically I think that, if the temperature is too low then, due to insufficient amounts of energy this will slow down the trypsin's speed of breaking down the gelatine and if the temperature is too high then it will start to kill off the enzyme which will also slow it down, so it needs to be somewhere in between. Preliminary test: I did this experiment previously to test whether the range of temperatures or the equipment needed to be changed. Here are the results of the preliminary testing: Temperature (?C). Time (secs). 30 1173 35 588 40 540 50 1188 Preliminary conclusion: These results are very spread out and therefore show that more temperature values will be required in the actual test. ...read more.

Conclusion

Leave the tubes for about 5 minutes so that they have a chance to warm up to their temperatures and then drop a strip of photographic film into each tube. Start a different stopwatch as the each strip goes in and label them so you do not get confused. Wait. Keep checking the baths at regular intervals and make sure that the entire protein layer from the film is gone before stopping the watches. Once all of the watches have been stopped, record the results, wash out the tubes and repeat twice. Once you have done the experiment three times then work out the averages and show the trend in a graph. Below I have drawn a diagram of how to set up the experiment. Safety: When using water baths be careful not to burn yourself and do not put the trypsin near your eyes or mouth. ...read more.

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