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To investigate the effect of pH on the ability of Catalase to breakdown Hydrogen Peroxide.

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Introduction

To Investigate The Effect of pH on the ability of Catalase to breakdown Hydrogen Peroxide Nature Of Problem NO2 and SO2 are dissolved in water in air to form nitric and Sulphuric acid decresing the soil pH. When water enters the crop as it is acidic it can affect catalase. Hypothesis The optimum activity will be at pH7,the activity should decrease on either side of the optimum. Prediction The catalase should work best at neutral pH tht is pH7,there it should have optimum activity. As pH curves are symmetrical it should decend on either side of the optimum. Changing the pH will break the bonds in the enzyme. ...read more.

Middle

Each amino acid making up a globular protein has a specific iso-electric point at which it carries no charge. The charges on amino acids cause attractive and repulsive forces within the globular protein and these help to give rise to tertiary structure. The active site is also charged. This is a result of the R groups of amino acids in the active site. Changes in pH may also change the charge of the R groups, decreasing the affinity between substrate and active site involves specific hydrogen and ionic bonds between enzyme R groups and the substrate. Each enzyme will only operate efficiently over a very narrow pH range. The optimum pH of different enzymes varies greatly depending on the environment in which they are to be used. ...read more.

Conclusion

The potatoes that I will use will be 1mm thick and 1cm wide. I will place ten potato chips in each test tube. I will then leave it into the solution for exactly for three mintes. Connected to the tube will be a rubber tube which will then be connected to a measuring cylinder filled with water. Oxygen will then move out of the rubber tube forcing the water to move out of the cylinder. When I will be measuring the concentrations of the solutions, the measuring cylinder must be read at the meniscus to avoid parallex. Results Table pH Oxygen collected (cm3) Test 1 Test 2 Test 3 Avarage 3 3 2 2 2.33 4 7 8 7 7.33 5 9 12 8 9.67 6 33 30 34 32.33 7 38 43 46 42.33 8 8 7 5 6.66 10 3 4 3 3.33 Discussion ...read more.

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