• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

What do you think will be the Relationship between the Concentration of Protease and the Rate of Breakdown of the Protein?

Extracts from this document...

Introduction

What do you think will be the Relationship between the Concentration of Protease and the Rate of Breakdown of the Protein? Hypothesis The higher the concentration of alkali Trypsin solution, the faster the breakdown of the protein casein. Null Hypothesis The less concentration of alkali Trypsin solution, the faster the breakdown of the protein casein. Variables Independent Variable * Enzyme concentration Dependent Variable * The absorption of light using a calorimeter. Controlled Variables * Temperature of the water bath �C * pH of enzyme. * Volume of Milk Powder. Apparatus * 25cm3 Milk powder - 5ml of milk powder (Casein) within each test tube. * Standard Alkali Trypsin Solution * 6 Test Tubes * Test Tube Rack * 2 Measuring Pipettes * Calorimeter * Stopwatch * Water Bath * Thermometer * Distilled Water * Buffer Method 1. In preparation to start the experiment make sure that the water bath is at 30�C. Having the water warm creates heat energy, which makes the substrate and the enzyme move faster, creating more collisions between the enzyme and the active site in the substrate, creating the product quicker. If the temperature is any higher than 40�C the enzyme will move so fast that it will start to loose it's globular shape, this means that the substrate will no longer be able to attach onto the active site so no more collisions will be made. ...read more.

Middle

Controlled Variable The temperature will be fixed at 30�C by using a water bath that will remain that temperature throughout. We will always use 5ml of milk powder, this and the Trypsin solution will then add up to 10ml, so will show how accurate the results are. Also I will be using a pipette, which will create more precision. The pH of the enzyme will remain the same as will be using a buffer to stop a change in the pH. I will combine the water with the buffer; this will be the buffer I'll be using to concentrate the Trypsin solution. Accuracy The equipment will bring precise and accurate results as the calorimeter will be within 0.01% and my pipettes, used to transfer my enzyme and substrate into the test tubes, will be within 0.1ml. Also as the water bath will be at a controlled temperature there won't be any chance of the temperature of the enzyme to decrease of increase, therefore creating a fair result. Safety * Wear goggles, in case the Trypsin or Casein solution goes in your eyes. * Make sure the pipette tip doesn't touch the desk as could contaminate it. Ethics No ethical concerns. ...read more.

Conclusion

I feel I needed to repeat the experiment, for each concentration about 5-10 times instead of just the once. That way I can take an average of my results and see if my first set of results are reliable and near a true value. If they are, they should be showing around the same results with each experiment. If I were to do the experiment again I would repeat the experiment 5-10 times for each concentration. I would also make sure I have enough time to get all results, this is because after 40mins I still did not get a result for the 1:4 concentration and this may make a difference to my results. If I got an error that was completely different again then I would repeat that experiment straight away, as it would have been an error I had made. Accuracy I don't think it is possible to say for definite whether my results were close enough to the true value. However I think that they were fairly accurate, as they appeared to go in the pattern, which I had predicted as it agrees with my hypothesis. Validity The systematical error has not made any significance to the results as they are still in a pattern, and the values are not to far out of range. ?? ?? ?? ?? Katrina Gillett ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Determine the effect of concentration on the activity of an enzyme Trypsin on the ...

    This procedure will be repeated with the enzyme called trypsin. However for trypsin, there will be a particular concentration. I will make use of the pipette by adding or removing few drops of the solution until they reach the required volume.

  2. We are going to find out how different enzymes (in this case, trypsin) concentration ...

    range, the rate of reaction would decrease (a enzyme starts to denature), but if extreme pH are reached then the enzyme would become completely denatured and can no longer carry out its function. Substrate concentration: Substrate concentration affect rate of reaction in a similar way to enzyme concentration, however, since

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work