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An investigation to determine the effect of concentration of protease, trypsin has on the rate of digestion of protein.

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Introduction

An investigation to determine the effect of concentration of protease, trypsin has on the rate of digestion of protein. First of all, the following equipments are needed: > A thermometer > A test tube > A beaker > crosses printed off from the computer > A measuring cylinder > A syringe > A stopwatch Trypsin is an enzyme which digests protein. In the following experiment, I will be investigating how the concentration of trypin affects the rate of reaction of protein digestion. Before setting up the experiment, there are a few variables that I need to control to make sure this is a fair experiment. > First of all, the temperature can change the rate of reaction. The more I increase the temperature, the faster the rate of digestion because more energy is put into the particles; therefore they have more energy to collide with each other, so they are more likely to contain the activation energy required for a successful reaction. It is also because the particles collide more often, so there is a bigger chance of collision. ...read more.

Middle

I can control the concentration of protein solution by using milk powder. I can either use the same solution I made for experiment, or mixed the same amount of milk powder with the same amount of water for each experiment. > The ph of the protein solution would need to be kept constant, as trypsin works better at a specific ph. Most enzymes are sensitive to ph levels and can become denatured by too low, or high ph. Trypsin is not an exception, it only works within a certain range of ph levels. The ph should be kept the same, by the use of a buffer, at its optimum level, which is around 7(according to the AS biology test book specification A). > The size and thickness of the cross that is used to stick on the test tube containing protein solution would need to be the same for every single experiment I do. > The only variable I am changing is the concentration of the enzyme (trypsin). ...read more.

Conclusion

* Measure 20cm3 of protein solution using a 20 cm3 syringe, again, this is accurate to �0.5 cm3 * Put the measured protein solution in a test tube. * Put the syringe, containing trypsin, and the test tube containing protein solution in the beaker for around 2 minutes for them to reach 30�C. (Use the thermometer to measure the temperature). * Print off several crosses and stick one on the test tube containing the protein solution. * Add the measured trypsin into the protein solution and start the stop watch simultaneously after 2 minutes. * The cross should not be visible through the solution at the moment. * When the protein has digested by trypsin, the colour of the solution will be clear and I will be able to see the cross through the solution. * Look at the cross through the test tube. * Stop the stopwatch when the cross can be seen. * Repeat experiment for each concentration of trypsin three times and take the result of the average. This can make the result more reliable. ?? ?? ?? ?? Alice Lee ...read more.

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