Does the concentration of sugar levels affect osmosis in potato plants?

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Simon Novakovic         Mrs LaBraca        Science c/work        Ecology

Does the concentration of sugar levels affect osmosis in potato plants?

Aim

To find out whether the concentration of sugar levels affect osmosis in a potato plant.

Introduction

Osmosis is the passage of water from a region of low sugar concentration to a region of higher concentration through a semi-permable membrane. Osmosis can happen also in the reverse ‘reverse osmosis’.

Diagram  

        Sugar solution

Petri dish

Potato bores

Method

 

        First collect all apparatus.

        Carefully drill six bores in a potato and cut length way’s circles, dab each slice dry.

 

        Weigh five disks till the total mass is exactly 3.2g, repeat the process six times and place five of the disks that’s mass is exactly 3.2g into the six individual petri dishes.

        Pour 20ml of each of the six sugar concentration solutions into the dishes and place the top on the petri dishes, this will there fore stop evaporation happening.

  Cautiously place the petri dishes in a sheltered area and leave for one day.

  Finally repeat the experiment two more times and collect results.

Prediction

I predict from my understanding of osmosis, water should pass from a region of low sugar concentration to a region of higher concentration through a semi-permable membrane, in this case the two guard cells. When water moves out of the two guard cells, pressure drops, they then become flaccid and the pores closes. I also understand osmosis happens in the reverse and that both forms of osmosis will happen in the experiment. There are six strengths of sugar concentration ranging from 0% (water) to 5, 10, 15,25 and 35%.

In the experiment I therefore predict that the potato slices in the lower sugar concentration solutions will have water moving into the lower epidermis, the two guard cells and the stomata pore. This will make the potato slices gain in mass; this is because water is passing to a region of low sugar concentration  (The solution) to a region of higher concentration (in the potato slices) through a semi permable layer (stomatal pore). Turgor pressure is being lost therefore the stomata is allowing water through.

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Consequently, I predict that the potato slices in the higher sugar concentration solution that the opposite will happen, water will move out of the stomatal pore, this will make the potato slices loss weight. This is because water will move from a region of high concentration (the potato) to a region of lower sugar concentration (the solution) through a semi permable layer (the lower epidermis, the stomatal pore) by using turgor pressure to push the water out.

         

Results table

Pilot experiment

Experiment 1

Experiment2

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