• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzyme Practical

Extracts from this document...

Introduction

ENZYME PRACTICAL Planning Hypothesis During my investigation I predict that there will be significant difference in the amount of reaction that takes place in the test tubes. I expect this because high acidic and alkaline conditions denature the enzyme, so I can therefore say that in the high PH solution the enzymes will not work very well and it will also not work well at the low PH concentrations. I can also say that catalyse activity will increase as the temperature increases up until an optimal point. At this point, as with most other variables it will either stay at this point or activity will fall. I predict, that the better conditions for enzyme catalysis will be around a PH of 7 (neutral), I just think this because water (which is neutral) makes up a large proportion of living things. Apparatus In my experiment I am going to need a range of apparatus of both materials and chemicals. Material wise I will need a 5cm syringe for injecting hydrogen peroxide into the boiling tube. I will require a boiling tube for the potato discs, 5cm of water and the hydrogen peroxide to go into to react in. ...read more.

Middle

Variables In my investigation there are a number of factors that could potentially affect my results, these can be from misreading results to having an unusual piece of potato. For my investigation to be fair I must keep the all the variables the same apart from one, which will be the PH buffer. I will vary this to find out the effect that PH has on enzyme activity. What I would not hope to do is to change any variables such as temperature because that would have a great effect on the results. Risk With the equipment that I will be using there will be certain risks, these include sharp edges, potential stabbing and poisoning. There is a lot of glass equipment that can smash or shatter and then have sharp edges, to avoid this I will take care not to drop, give impact or crush (over tightening of the clamps) the glass ware e.g. boiling tube, burette. The needle can also give a high risk of stabbing since it has a very sharp metal point, to avoid this I will take as much care to avoid it when I replace the rubber bung after refilling the boiling tube with potato's and PH buffer. ...read more.

Conclusion

As the graph does show when there is a low PH of 3 there is little catalytic activity but then as the PH goes up to around 7 the activity increases but then reaches its optimum point and then activity falls again as the enzyme is denatured. Explanation From my experiment and my biological knowledge of enzymes I can conclude that. Temperature affects enzymes in a way that at high temperatures the enzymes and substrate molecules have high kinetic energy. Which basically means is that that the molecules are moving around more in the solution and therefore collide more often and the substrate and enzymes can react quicker. Also with high concentrations of both acids and alkalises the enzymes denature and as the intensity of the PH's increases it becomes ever more difficult for the substrates to fit into the active site, this slows down or at high extremes stops the reaction. The concentrations of enzymes and substrate molecules also has an effect on the reaction time and amount, a reaction can only go so far if there is not an equal measure of both. If there was 500 enzyme molecules and only 100 substrates then the reaction would only take place up until all the substrates have formed enzyme-substrate molecules, so therefore 400 enzymes will be unused. Page 4 of 4 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work