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How does the concentration affect the enzyme action of catalase.

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Introduction

Catalase is a very fast reacting enzyme, it is found in many living cells, it breaks down hydrogen peroxide to water and oxygen. One molecule of it can deal with six million molecules of hydrogen peroxide in 1 minute. Hydrogen peroxide is toxic so needs to be changed into harmless substances. Catalase break down Hydrogen peroxide water + oxygen 2H2O2 2H2O + O2 I have been set the task of finding out how the surface area affects the rate of catalase action. In my preliminary testing I found out which size of measuring cylinder to use for the final test and what condition would be the best. Whilst all of this I had to make the test as fair and accurate as I could by measuring all the liquids and measuring of potato accurate. I have carried out some preliminary testing to experiment how the surface area affects the rate of reaction. I have chosen to have a time limit of 10 minutes and 20cl of peroxide. The purpose of these prelims was to see if one potato cube (at 6cm squared) was the same as five potato cubes (30cm squared). ...read more.

Middle

Then put the hydrogen peroxide in the measuring cylinder place bung on to stop the oxygen being lost. Then start the stop clock. I used 20ml of hydrogen peroxide each time with a paper towel around every side arm test tube. I will time each test for six minutes. The first test will be with 1 potato cube in with 20cl of hydrogen peroxide this one test will be repeated 3 times the all the other test will have all same conditions except more potato cubes (more surface area). All the results I found were put into a table the transferred on this work the results are shown below. Results from experiment 1 potato cube Surface area cm2 Volume of o2 produced 6cm2 4.2 ml 6cm2 5.1ml 6cm2 4.5ml 2 potato cubes Surface area cm2 Volume of o2 produced 12cm2 10.5 12cm2 11 12cm2 10.5 3 potato cubes Surface area cm2 Volume of o2 produced 18cm2 12 18cm2 13 18cm2 13.5 4 potato cubes Surface area cm2 Volume of o2 produced 24cm2 15.5 24cm2 16 24cm2 16.5 5 potato cubes Surface area cm2 Volume of o2 produced 30cm2 18.5 30cm2 18.5 30cm2 19 Averages table (table of results final) ...read more.

Conclusion

Any that will also make the particle move more when they should. I tried to stop people from banging into the desk to prevent this. Always use different paper towels at the start of each experiment to prevent different temperatures. Always use 20 vols of hydrogen peroxide Always wear safety goggles to protect the eyes from the hydrogen peroxide. Keep all apparatus away from the edge of the desk to prevent breakages. Do not run around or rush about. I think that my experiment went fairly well but as I predicted that the increasing of surface area would increase the rate of reaction. This was clearly shown by the result tables I have made. If I would have had more time then I would have made more results and made more averages to make the experiment more accurate. And would of tried to solve how to stop the potatoes from clumping together as this was a problem because there was less surface area Equipment used: * Side arm test tube * 2 x 25cl measuring cylinder * water bath * stand and clamp * stop clock * hydrogen peroxide * paper towels * potato * cutting tile * scalp knife * rule Diagram of equipment: DANIEL BIRCHALL BIOLOGY COURSEWORK ...read more.

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