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How does the temperature of catalyse affect the rate of which it reacts with hydrogen peroxide?

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Introduction

How does the temperature of catalyse affect the rate of which it reacts with hydrogen peroxide? Aim The ain of this investigation is to find out how the temperature of the enzyme catalyse affects the rate that it reacts with hydrogen peroxide Prediction I predict that the higher the temperature of the catalyse, the quicker the reaction with hydrogen peroxide but when the temperature of the enzyme excides about 40 C will start to decrease. I think this because of my knowledge about enzymes. I know that enzymes are found in all living things. I have found out when doing previous experiments with enzymes that the optimum temperature for a reaction involving enzymes is about 40 C after about 40 C the bonds in the enzyme begin to brake or denature this makes the enzyme loose its shape and stop working so the reaction rate will decrease and eventually the reaction will stop. Equipment list To carry out the following investigation I will need: o Safety goggles o Clamp and stand o A boiling tube o Measuring cylinders o Delivery tube o Water bath o Bowl of water o Hydrogen ...read more.

Middle

Attach the dilivery tube and place end of the dilivery tube under the und of a measuring cylinder, which is filled with water and upside down in a bowl of water. o Start stop watch and time for 1 minute o At the end of 1 minute records the amount of gas given off o Repeat this experiment for 10 C 20 C 30 C and 50 C o Repeat the entire investigation at lease 1 more time so that I can get an average y doing this I reduce my margin of error Diagram Preliminary work From preliminary work that I have carried out I have found out that I will have 2 use 20 cm3 of both hydrogen peroxide and yeast to get a measurable amount of gas and also changing the amount of time I let the reaction go on for before taking the results I will reduce to 1 minute. Results Temperature ( C) Gas given off in 1 minute Experiment 1 Gas given off in 1 minute Experiment 2 Gas given off in 1 minute Average of experiments 1 and 2 10 C 2.1 1.9 2.0 ...read more.

Conclusion

Evaluation I am pleased with what I achieved in the investigation. I believe that my experiment was as accurate as I could make it as it was quite difficult to make sure that the temperature of the enzyme was exactly the temperature need as the water baths would cool down quite quickly and I would have 2 top it up with warmer water which changes the temperature of the enzyme by a few degrease. Another problem I found was with the upside down measuring cylinders to measure off the amount of gas given off, as sometimes I would get small air bubbles before I started the experiment so this affected my results very slightly. So if I did this experiment again I would probably use a gas syringe, as this would give me a more accurate reading. But I don't think these problems had a major impact on the quality of my results because the amount of error was minimal. Further work that I could do to is to investigate the how Ph effects the rate of reaction of catalyze and hydrogen peroxide. Claire Bartlett (set 1) ...read more.

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