How does the temperature of catalyse affect the rate of which it reacts with hydrogen peroxide?

To carry out the following investigation I will need:

• Safety goggles
• Clamp and stand
• A boiling tube
• Measuring cylinders
• Delivery tube
• Water bath
• Bowl of water
• Hydrogen peroxide
• 0.5 yeast
• Thermometer
• Stop watch

Variables

• The amount of yeast and hydrogen peroxide needs to be the same throughout the experiments so I will chose an amount that I can use throughout the experiments.

• The temperature of hydrogen peroxide will b 2) C when the yeast is added to stop the temperature of the hydrogen peroxide being a contributing factor to reaction rate

• For each run of experiment is will only use yeast and hydrogen peroxide from the same batch

• The only variable that I will change will be the temperature of the yeast.

Plan

• Put 2 cm3 of 0.5 yeast into a boiling tube
• Place that boiling tube into a water bath that is heated to 40 C
• Wait until the yeast also reaches the temperature of 40 C (start at 40 because this is the temperature that I expect to have the most reaction so if I don’t get a good result here I will have 2 change my plan
• Then add 2 cm3 of hydrogen peroxide to the yeast and place in the clamp
• Attach the dilivery tube and place end of the dilivery tube under the und of a measuring cylinder, which is filled with water and upside down in a bowl of water.
• Start stop watch and time for 1 minute
• At the end of 1 minute records the amount of gas given off
• Repeat this experiment for 10 C 20 C 30 C and 50 C
• Repeat the entire investigation at lease 1 more time so that I can get an average y doing this I reduce my margin of error

Diagram

Preliminary work

        From preliminary work that I have carried out I have found out that I will have 2 use 20 cm3 of both hydrogen peroxide and yeast to get a measurable amount of gas and also changing the amount of time I let the reaction go on for before taking the results I will reduce to 1 minute.

Results

Graph

Analysis

From this investigation I can see that my prediction was correct. The investigation conformed my thought that that the rate of reaction will increase as the temperature rises. The investigation also shows that as the temperature raised by 10 C the rate or reaction doubled it also showed that as I said in my prediction the enzyme started to de nature at about 40 C

        My results can be explained by the kinetic theory which is when particles gain heat energy they begin to move quicker. This meant that catalyse particles collide with the hydrogen peroxide more often and so causing more reaction so as the temperature rises the reaction rate becomes greater. Also I can explain the reason why the rate of reaction starts to decrease at around 40 C. This is because at around 40 C enzymes are denatured. This is then the bonds keeping the shape of the enzyme begin to brake and so the enzymes shape changes and is unable though work on the specific substance.

Evaluation

I am pleased with what I achieved in the investigation. I believe that my experiment was as accurate as I could make it as it was quite difficult to make sure that the temperature of the enzyme was exactly the temperature need as the water baths would cool down quite quickly and I would have 2 top it up with warmer water which changes the temperature of the enzyme by a few degrease. Another problem I found was with the upside down measuring cylinders to measure off the amount of gas given off, as sometimes I would get small air bubbles before I started the experiment so this affected my results very slightly. So if I did this experiment again I would probably use a gas syringe, as this would give me a more accurate reading.

But I don’t think these problems had a major impact on the quality of my results because the amount of error was minimal.

Further work that I could do to is to investigate the how Ph effects the rate of reaction of catalyze and hydrogen peroxide.