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In this experiment I am going to try and find out the factors that affect enzyme controlled reactions by using the Catalase in potato.

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Introduction

Biology GCSE Coursework: Planning In this experiment I am going to try and find out the factors that affect enzyme controlled reactions by using the Catalase in potato. Enzymes are non-living and act as biological catalysts, which speed up reactions. They can change shape if the temperature changes. Enzymes are made up of active sites (the lock) and only certain shaped substrates can fit into them to cause a chemical reaction. To work out this experiment, I am going to use a formula of: 2H2O2-using catalase to become 2H2O+O2 I will use a set of five pH's to get my readings from the collected oxygen. The amount of oxygen is directly related to the rate of catalase activity. ...read more.

Middle

will always be 5cm3 each time, the tests will all be done at room temperature, the tests will be repeated to get an average and my weight of potato will be the same for each of the experiments. Potato is good substance because it contains many active sites to react with. I will be changing the pH in order to measure the effects on the rate of catalase activity. In this experiment the five pH's I will be using are: pH2, pH4, pH6, pH8 and pH 10. At low pH's the enzymes are denatured because the pH interferes with the bonding of the amino acids and the shape of the active site is changed. ...read more.

Conclusion

Above that pH the enzyme starts to denature (change in shape). The enzyme changes shape because heat causes to many vibrations inside the enzyme Therefore the active site changes shape; the substrate no longer fits into the active site, so the rate of reaction decreases and is slow. To increase the accuracy of my investigation I will create a detailed plan, have several diagrams and note down a risk assessment. The potato size will have to be small enough to be completely submerged in hydrogen peroxide (the substrate). . After doing my preliminary test I discovered that if the potato was measured in length then this could be inaccurate e.g. dents. Doing a preliminary test before means I will know how to set up equipment and do the experiment, this will mean I make fewer mistakes. There are several factors which are difficult to control ...read more.

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