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Investigate the effect of changing the hydrogen peroxide concentration on the rate of reaction of the break down of hydrogen peroxide by catalase.

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Introduction

Planning Sheet for Science Investigation Problem/Aims My aims for this experiment are to investigate the effect of changing the hydrogen peroxide concentration on the rate of reaction of the break down of hydrogen peroxide by catalase. Background Information Predictions I predict that the higher the concentration of hydrogen peroxide is the more it will react with the catalase and therefor producing more oxygen. This is due to the fact that as the concentration of hydrogen peroxide is increased, the number of collisions between the enzyme (yeast) and substrate (hydrogen peroxide) increases. I believe that if I double the concentration of hydrogen peroxide, the rate of reaction will also double. Key Factors *The main factors that I fell will influence the experiment are: 1) Temperature of the catalase and hydrogen peroxide. As the more heat there is the more kinetic energy the enzyme and substrate will have causing them to move faster which will then cause more collisions and faster reactions rates. 2) The concentration of hydrogen peroxide. The higher the concentration of hydrogen peroxide the more substrates there will be, which will enable more collisions between the hydrogen peroxide and catalase, therefore the reaction rate will increase. ...read more.

Middle

This is where the reaction of the break down of hydrogen peroxide by catalase is going to take place. 9) I placed the conical flask in a large water container. This is going to be filled with water and have a thermometer in it. This will act as a water bath to try and keep the temperature of the solutions constant. 10) The burette is turned upside down and clicked on to it's stand, this is where the oxygen produced from the reaction is gong to be collected and record. This is to help me find out how much oxygen is produced in two minutes. (I must make sure the bottom of the burette is closed completely to make sure no oxygen will enter or escape.) 11) The opening of the burette will stand in another container of water. I must make sure the water level is over the top of the opening of the burette or my results will not be reliable. The burette must be filled with water, but again I must make sure the water stops on one of the markers so I can make an exact result of where the starting measurement of the water in the burette will be. ...read more.

Conclusion

To find out the process data I used the same formula as in table one, which is; Starting measurement - 5cm3 - Final measurement = Total oxygen produced Beaker number Concentration of H2O2 / M Volume of h202 / cm3 Volume of distilled water / cm3 Volume of catalase used / cm3 Starting measurement / cm3 Final measurement /cm3 Total oxygen produced / cm3 1 1.0 5 0 5 48.2 29.6 13.6 2 0.9 4.5 0.5 5 46.3 28.3 13.0 3 0.8 4 1 5 47.1 29.3 12.8 4 0.7 3.5 1.5 5 49.3 31.9 12.4 5 0.6 3 2 5 48.7 32.4 11.3 6 0.5 2.5 2.5 5 47.1 31.3 10.8 7 0.4 2 3 5 48.7 34.3 9.4 8 0.3 1.5 3.5 5 48.4 36.6 6.8 9 0.2 1 4 5 47.3 38.8 3.6 10 0.1 0.5 4.5 5 49.7 43.9 0.8 11 0.0 0 5 5 47.3 42.3 0.0 Table 3 - this table shows the averages of tables 1 and 2 on the total oxygen produced for each concentration of hydrogen peroxide. Number Beaker Concentration of H202 / cm3 Average total oxygen produced / cm3 1 1.0 14.0 2 0.9 13.4 3 0.8 13.0 4 0.7 11.8 5 0.6 11.1 6 0.5 10.4 7 0.4 9.3 8 0.3 6.9 9 0.2 3.8 10 0.1 1.1 11 0.0 0.0 *Graph - ...read more.

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