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Investigating the breakdown of hydrogen peroxide by catalyse.

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Introduction

Introduction For this coursework I have to investigate an enzyme reaction, the reaction that I will be investigating will be the breakdown of hydrogen peroxide (which is a poison) by catalyse (which is the enzyme). When is is broken down the result products of this reaction are water (h2o) and oxygen (o2). In my coursework experiment, I am going to investigate the amount of oxygen produced when a source of catalase enzyme is added to hydrogen peroxide, H O . In my experiment, I am going to focus on how the concentration of the peroxide affects the rate of reaction i.e. the concentration goes up, the rate of reaction increases. The catalyst is one, which makes the peroxide decompose into water and oxygen. An enzyme is a biological catalyst. They speed up the rate of a reaction however they are not affected themselves whilst doing this, this is why they are catalysts. Each enzyme has an active site. 2H2O2 2H2O + O2 Hydrogen Peroxide Water + Oxygen Variables-in this investigation there are several factors that will effect the out come of the experiment. They are: 1. the amount of hydrogen peroxide 2. the size of the cork borer 3. the time inbetween measureing the oxygen 4. amount of potatoe (catalayse) In my experiment I will change the amount of potato (catalyse) ...read more.

Middle

produced (ml) number of pieces of potato 1mins 2mins 3 mins 4mins 5mins 6mins 7mins 8mins 2 4 6 6 7 8 8 8 9 3 1 5 7 9 10 10 10 11 5 5 7 12 15 17 20 20 20 6 9 15 18 21 24 26 26 27 8 4 10 13 16 17 19 21 21 10 10 17 22 25 29 31 35 35 12 13 21 29 32 37 38 39 41 Results tables First experiment Second experiment volume of gas (o2) produced (ml) number of pieces of potato 1mins 2mins 3 mins 4mins 5mins 6mins 7mins 8mins 2 3 4 4 4 4 4 6 8 3 4 7 9 10 11 12 13 14 5 7 10 12 13 14 15 16 16 6 10 16 18 18 21 25 26 28 8 9 12 14 15 16 17 17 18 10 7 10 13 16 20 21 22 23 12 14 22 30 31 36 39 40 41 Third experiment volume of gas (o2) produced (ml) number of pieces of potato 1mins 2mins 3 mins 4mins 5mins 6mins 7mins 8mins 2 1 4 4 4 4 4 6 7 3 4 6 6 7 9 10 11 12 5 10 16 18 21 23 26 26 25 6 10 16 18 18 21 25 26 28 8 12 16 19 20 22 25 26 27 10 10 ...read more.

Conclusion

In the graph most of the results were accurate but there are a few anomalous points (they are circed on my graph). This couldn't have been caused by the concentration of the H2O2 could not have been the problem here because all the other results that used the H2O2 from the same bottle were roughly on target. It could have been caused by a change in temperature but this is unlikely because most of the other results that were recorded at the same time fitted in with the trend. Probably the thing that caused this result to be anomalous was that there may have been an abnormal bit of potato. As I explained in the concultion the ratio of H2O2 and potato was different in each experiment because the same amount of H2O2 was used in each experiment. Therefore the supply of the H2O2 may have started to run out towards the end of the experiments with more potato and cause the results to change. What could be done to prevent this would be putting the same ratio of H2O2 to potato in each experiment or putting the pieces of potato in a larger solution of H2O2 so that the supply does not run out. One other problem that might have affected the experiments was that the pieces of potato might have been touching each other and blocking each other's access to H2O2. ...read more.

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