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Producing a pure culture of bacteria form a mixture.

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Introduction

Producing a pure culture of bacteria form a mixture. Ishita Patel 09/10/02 The bacterium that we are sub culturing is called Bacillus Subtilis. The aim of the experiment is to isolate pure cultures, as individual colonies. Apparatus: Two sterile petri dishes 1) containing a pre-set layer of sterile nutrient agar 2) An unfilled, sterile petri dish The petri dishes used to subculture bacteria are disposable. Inoculating loop Bunsen Burner Disinfectant solution, i.e. Dettol Paper towels "BIOHAZARD TAPE" Marker pen Fresh, warm (50'c - 60'c), sterile nutrient agar. ( Agar is a mixture of seaweed and nutrients) Aseptic techniques: Thought out the experiment we followed various aseptic techniques. The aim of these aseptic techniques was to avoid contamination from other sources of bacteria, i.e. pathogens These bacterial may not be initial harmful, however if they enter our petri dish, they may find the conditioned to grow, results in; 1) ...read more.

Middle

Also write the information at the edge of the dish, so that it is possible to still see the bacteria, after growth, clearly. I set up a �M ��� bjbj�=�= .&?W?W�������l�������F�M ��� bjbj�=�= .&?W?W�������l�������F&F" 2FFFFFFFF� � � � � � � $T t�� �FFF[0]FF� ���FF� ���F:�F�F� �F� �@�* -U ��� F: ?�?�o�F�?jm � � 0" u ,4�4� � .�����Producing a pure culture of bacteria form a mixture. Ishita Patel 09/10/02 The bacterium that we are sub culturing is called Bacillus Subtilis. The aim of the experiment is to isolate pure cultures, as individual colonies. Apparatus: Two sterile petri dishes 1) containing a pre-set layer of sterile nutrient agar 2) An unfilled, sterile petri dish The petri dishes used to subculture bacteria are disposable. Inoculating loop Bunsen Burner Disinfectant solution, i.e. ...read more.

Conclusion

This is not the optimum temperature of the bacteria, infact the optimum temperature for the bacteria would b 35'c-45'c. However this is dangerous as this is also the optimum for many human pathogenic bacteria. And if they have some how entered the plate they could begin to cultivate. Human bacteria have , however a narrow temperature range for there optimum, and so we can use a close enough temperature to the optimum for the bacteria, and this temperature would not act as a n optimum for the human bacteria. Also turn the plate upside down, so the lid acts as the base. So that as the warm agar causes condensation within the closed petri dish, the water droplets don't fall onto the bacteria, and cause any individual colonies of bacteria to merge together. By now, your agar should have set. Repeat the steps mention above following the same aseptic technique. However this time when streaking the plate simply swipe the inoculating loop across the agar. ...read more.

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