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The aim of this experiment is to find out how the substrate concentration effects the action of lipase.

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Introduction

Robynne Shipley 10G Factors Effecting Action of Lipase Effect of Substrate Concentration on the action of Lipase Aim The aim of this experiment is to find out how the substrate concentration effects the action of lipase. Method * 4 test tubes were taken and put into a test tube rack. * Each test tube was labelled using (full fat; semi (skimmed); jersey (full fat); skimmed) with the type of milk that was going to be put into it. * 5 cm� of milk was put into each of the test tubes. * 7 cm� of sodium carbonate was then put into each of the test tubes and a few drops of phenothalin. * Finally 1 cm� of 5% conc. Lipase was added and the stopwatch started. * Each time one of the solutions lost its colour (i.e. ...read more.

Middle

(No result) N/A Analysis The results and the graph clearly show that as the substrate concentration increases the time decreases. Therefore this has proved my prediction to be wrong. I predicted that as the substrate concentration increased then so would the time, because at the time I believed that the more fat particles there was the longer the reaction time would be. But my experiment has proved my wrong, and it has made clear that the more fat particles there are the quicker the rate of reaction. This is because there are more fat particles for the lipase to react with and therefore more chance of a collision between the two so more chances of reaction and so the reaction is faster. And like in the skimmed where there are far fewer fat particles than in other milks there is less chance of a collision and so the rate of reaction is slower. ...read more.

Conclusion

If I could do this experiment again I would take more time over it and I would like to see how long it would actually take for the skimmed milk to react. But I could try to speed this up by using bile salts in all of the different types of milk, as bile salts break down the fat particles into smaller pieces and therefore make it easier for the lipase to digest, I am aware that there are not many fat particles in the skimmed milk anyway but if the bile salts could brake down whatever there already is then there would be more particles for the lipase to react with. Of course the bile salts would have to be added to all of the test tubes and so they would all react much quicker as well. But that is not a problem, as it would just speed up the time the experiment takes and therefore save time in the end. ...read more.

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