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The effect of concentration on the activity of catalase.

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Introduction

Title: The effect of concentration on the activity of catalase. Brief Introduction: This experiment will look at the effect of varying the concentration of the enzyme, catalase on its performance. Potato will be the source of catalase. Hydrogen peroxide is a toxic substance that may be formed during aerobic respiration. It must be removed from the system and catalase breaks it down into harmless substances. The equation for the reaction is: 2H2O2 --> 2H2O + O2 Hypothesis: Increasing the concentration of the enzyme, catalase will increase the volume of oxygen produced per unit of time. The nature of the problem to be investigated is the factor that is effecting the rate of the reaction and what effect this factor will have on the rate of reaction when it is increased. In this investigation, the factor effecting the rate of the reaction is the concentration of the enzyme. Increasing the concentration of the enzyme, catalase will increase the volume of oxygen produced per unit of time. The reason for this is as follows. For a chemical reaction to take place, the substrate molecules must collide with the enzyme molecules and to be specific, must result in a successful collision. This is called the collision theory. Increasing the enzyme concentration will increase the number of molecules. This will increase the frequency of collisions and increase the chance of successful collisions. This will in turn increase the rate of the reaction. Null Hypothesis: Increasing the concentration of the enzyme, catalase will have no effect on the volume of oxygen produced per unit of time. Aim: To investigate the effect of varying the concentration of the enzyme, catalase, on the rate of reaction. The volume of oxygen produced per unit of time will measure to indicate the rate of the reaction. Prediction: The prediction for this investigation is that the volume of oxygen produced per unit of time increases with the increasing concentration of catalase. ...read more.

Middle

That is to say that 2cm3 catalase had a greater number of molecules then 1cm3, 3cm3 had a greater number of molecules then 2cm3 and so on. This increased the frequency of collisions, the occurrence of successful collisions and subsequently the rate of the reaction. To be more specific, the differences in the rate of reaction can be explained by what effect increasing the enzyme concentration had on the rate of reaction. Figure 2 shows how an enzyme works. The 5cm3 catalase had the greatest enzyme concentration so it had the greatest number of active sites. Due to the surplus number of active sites, the enzyme is performing at its optimum. The 5cm3 catalase had the greatest rate of forming enzyme substrate- complexes, which resulted in the formation of the products and therefore the greatest rate of reaction. 1cm3 catalase had the lowest rate of reaction because it had the least number of active sites and this meant that the enzyme was not performing at its optimum. The turnover of enzyme-substrate complexes was lowered because of this. The rate of reaction increased with increasing concentration of catalase because as the concentration increased there was an increased number of active sites. This meant that the enzyme was performing at its optimum. The turnover of enzyme-substrate complexes increases with increasing concentration and this increases the rate of reaction. This is sufficient evidence to disprove the null hypothesis. The results are reliable and can be used to disprove the null hypothesis as the graph indicates that there is a strong correlation between the concentration of catalase and volume of oxygen produced. There are no anomalies or inconsistencies in the results. The points are all close to the line of best fit and conform to the pattern of the results. All variables from the pilot experiment will be maintained in the final experiment. Final Experiment Aim: To investigate the effect of varying the concentration of the enzyme catalase, on the rate of reaction. ...read more.

Conclusion

2.0cm3 14cm3 36cm3 90cm3 122cm3 270secs 2.2cm3 17cm3 40cm3 102cm3 137cm3 300secs 2.5cm2 18cm3 46cm3 114cm3 151cm3 Final Results: Trial 1 Volume of potato 1cm3 2cm3 3cm3 4cm3 5cm3 Time 30secs 0.4cm3 3cm3 7cm3 15cm3 22cm3 60secs 0.7cm3 6cm3 11cm3 26cm3 37cm3 90secs 0.8cm3 8cm3 14cm3 37cm3 44cm3 120secs 0.9cm3 10cm3 16cm3 47cm3 53cm3 150secs 1.2cm3 11cm3 19cm3 59cm3 68cm3 180secs 1.5cm3 14cm3 22cm3 70cm3 82cm3 210secs 1.8cm3 16cm3 26cm3 83cm3 96cm3 240secs 2.0cm3 18cm3 30cm3 94cm3 110cm3 270secs 2.3cm3 21cm3 35cm3 106cm3 125cm3 300secs 2.5cm3 22cm3 40cm3 117cm3 154cm3 Trial 2 Volume of potato 1cm3 2cm3 3cm3 4cm3 5cm3 Time 30secs 0.5cm3 1cm3 6cm3 15cm3 21cm3 60secs 0.6cm3 3cm3 8cm3 25cm3 36cm3 90secs 0.8cm3 5cm3 11cm3 36cm3 51cm3 120secs 0.9cm3 6cm3 14cm3 47cm3 66cm3 150secs 1.2cm3 9cm3 17cm3 58cm3 80cm3 180secs 1.4cm3 11cm3 20cm3 70cm3 94cm3 210secs 1.5cm3 15cm3 24cm3 82cm3 119cm3 240secs 1.7cm3 16cm3 29cm3 94cm3 124cm3 270secs 1.9cm3 20cm3 32cm3 105cm3 132cm3 300secs 2.2cm3 22cm3 36cm3 117cm3 140cm3 Mean Value of Oxygen produced Volume of potato 1cm3 2cm3 3cm3 4cm3 5cm3 Time 30secs 0.45cm3 2cm3 6.5cm3 15cm3 21.5cm3 60secs 0.65cm3 4.5cm3 9.5cm3 25.5cm3 36.5cm3 90secs 0.8cm3 6.5cm3 12.5cm3 36.5cm3 47.5cm3 120secs 0.9cm3 8cm3 15cm3 47cm3 59.5cm3 150secs 1.2cm3 10cm3 18cm3 58.5cm3 74cm3 180secs 1.45cm3 12.5cm3 21cm3 70cm3 88cm3 210secs 1.65cm3 15.5cm3 25cm3 82.5cm3 107.5cm3 240secs 1.85cm3 17cm3 29.5cm3 94cm3 117cm3 270secs 2.1cm3 20.5cm3 33.5cm3 105.5cm3 128.5cm3 300secs 2.35cm3 22cm3 38cm3 117cm3 147cm3 SINTHUJA SATHIANATHAN THE EFFECT OFVARYING THE CONCENTRATION OF CATALASE ON THE RATE OF REACTION. Contents Introduction 1 Background Knowledge 2 Pilot Experiment * Aim 5 * Apparatus with justification 5 * Safety 5 * Variables 6 * Procedure 6 * Summary Table 7 * Conclusion 7 Final Study * Aim 9 * Apparatus and justification 9 * Safety 9 * Variables 10 * Procedure 10 * Summary Table 11 * Conclusion 11 * Evaluation 13 Appendix 15 Pilot results 16 Final Results 16 Mean results of final experiment 17 Raw data Error bars ?? ?? ?? ?? 1 1 ...read more.

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