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The effect temperature has on the rate of anaerobic respiration in yeast.

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Introduction

The effect temperature has on the rate of anaerobic respiration in yeast. Aim: To investigate the effect temperature has on anaerobic respiration in yeast. Prediction: I predict that as the temperature of the water rises the faster the enzymes in the yeast will break down the glucose, therefore producing more carbon dioxide and more anaerobic respiration will take place. But when the temperature gets above at least 72c I predict that the enzymes in the yeast will stop working and there will be a decrease in the amount of carbon dioxide being produced and less anaerobic respiration taking place. Anaerobic respiration is respiration without oxygen. Yeast can respire with or without oxygen in this case the yeast is without oxygen. When it respires without oxygen it is called fermentation. The chemical formula is Glucose Alcohol + Carbon Dioxide + Energy. Because of my scientific knowledge I know that yeast enzymes only ferment in sugar and flour (we are using sugar). I also know that temperature effects the rate at which enzymes work, making them move and work faster on the substrate (in this case is sugar), because heat produces energy. The diagram below shows the lock and key theory of enzymes the substrate has to fit exactly into the enzyme other the substrate can not be broken down. ...read more.

Middle

* PH also effects the rate at which enzymes work. I am using water, which is neutral, so this will not effect the enzymes and the pH will stay the same through out the experiment. Results: Room Temperature (25c) 37c 50c 80c 1 5:40 4:30 3:35 Didn't get to 2.5ml 2 5:50 4:40 3:40 Didn't get to 2.5ml 3 5:60 4:50 4:00 Didn't get to 2.5ml Average 5:50 4:40 3:58 Didn't get to 2.5ml Conclusion The results from my test clearly show me that, the higher the temperature of the water bath the faster the anaerobic respiration takes place and more carbon dioxide is produce, until the temperature gets to 80c were the anaerobic respiration stops working. From my scatter diagrams I can see that there is a vast in increase in carbon dioxide being produced when the heat is increased because the line of best fit has fairly strong negative correlation. I tried to find a trend or a pattern within the average graph. Using my line of best fit I found out how much time it took to produce 2.5ml of carbon dioxide for every 10c. Here is the table of results for every 10c. 10c 20c 30c 40c 50c 60c 70c 80c 6.42 mins 6.04 mins 5.24 mins 4.46 mins 4.06 mins 3.27 mins 2.48 ...read more.

Conclusion

The bubbles leaving the syringe were actually oxygen because the yeast and glucose solution was at the back of the syringe so the carbon dioxide was pushing the oxygen out of the syringe. This doesn't effect the test, as the amount of oxygen leaving the syringe was equivalent to the carbon dioxide being produced. I used as much computer technology as possible to ensure accurate results. I used a stopwatch and an electronic water bath. My evidence from my results was sufficient to support my conclusion because it clearly proved the amount of carbon dioxide produced increased as the temperature increased. My scatter diagrams, line of best fit and tables ensured that the evidence was clear although I thought I could have used more than one type of graph to show my evidence. To improve my investigation I would have used a wider verity of graphs and charts to show my results. I would have tested the pH of the water bath before each test, so I knew it was neutral. I would do this because pH is another factor like heat that effects enzymes rate of work. I would like to use to do another investigation exactly the same as this one but using the variable as pH instead of temperature. Maybe then the experiment would give clear results, that show more patterns and trends that are easier to analyse and understand. ...read more.

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