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To find out the effects of changing the surface area of a potato chip has on the rate of reaction between catalase and hydrogen peroxide.

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Aim: To find out the effects of changing the surface area of a potato chip has on the rate of reaction between catalase and hydrogen peroxide. Background Information: The catalase, which is an enzyme, is found in the cells of plants and animals so potato will be used in this experiment. It breaks up hydrogen peroxide into water and oxygen. An enzyme is a chemical, which makes a reaction happen more quickly, they are also known as biological catalysts. Enzymes can be affected by pH, temperature or the concentration of the enzyme or the substrate. Basic Plan: In the preliminary test, measure the height of foam in different temperatures every minute, to see which temperature the experiment should be kept at. In the main experiment we are going to measure the volume of gas given off every 30 seconds. Factors and Fair Testing: The temperature the solution has an affect on the experiment. The higher the temperature of the solution, the quicker the reaction, until it reaches a certain point. Then the rate of reaction will then decrease. This is due to the kinetic energy of the particles within the solution. ...read more.


This is due to the increase of enzymes in the same amount of volume and therefore the more active sites available, the quicker the substrate (hydrogen peroxide) can be broken down. The surface area of the potato would affect the experiment due to the increase of catalase on the outside of the potato for the hydrogen peroxide to react with. As the catalase is contained within the cells, the more cells that are exposed to the hydrogen peroxide, the more enzymes there are to react with. The number of active sites increases and this increases the number of enzyme substrate complexes, which then release more hydrogen. This is the factor we are going to use in our experiment and change the length of the potato chip with the width remaining the same. Equipment List: * 5 test tubes * Test tube rack * Knife * Potato chips (width and depth of 1cm) * Measuring cylinder * Hydrogen peroxide 6% concentration * Goggles * Stopwatch * Delivery tube * Tub full of water * Measuring cylinder Method: 1. Set up the equipment as in the diagram above. 2. Cut up 5 strips of potato, one strip 1cm long, one strip 2cm long etc. (All strips have a width of 1cm) ...read more.


2.6 3.0 2 1.5 3.5 4.5 5.0 6.5 7.0 7.6 8.5 9.4 10.0 3 1.5 3.5 4.5 5.5 6.5 7.5 8.5 9.4 10.3 11.0 4 2.4 3.5 5.4 6.5 8.0 9.0 10.5 11.5 12.5 13.5 5 5.0 6.0 8.5 10.5 12.0 13.5 15.0 16.5 17.5 19.0 3rd attempt 1 2 2.0 2.5 3.5 4.2 5.0 5.5 6.5 7.0 7.5 8.0 3 1.5 3.0 4.5 5.0 5.0 6.0 7.0 8.0 8.5 9.5 4 3.0 3.5 5.2 6.4 7.5 8.5 9.3 10.5 11.4 12.2 5 4.0 5.5 7.0 8.0 9.4 10.5 11.5 12.5 13.5 14.5 Average Set (used for graph) Average Length of Potato (cm) Volume of Gas (cm�) (correct to decimal places) 0.30 minutes 1 minute 1.30 minutes 2 minutes 2.30 minutes 3 minutes 3.30 minutes 4 minutes 4.30 minutes 5 minutes 1 0.77 0.93 1.50 2.17 2.93 3.20 3.67 3.67 4.53 5.00 2 1.50 2.67 3.50 4.15 5.17 5.58 6.37 7.00 7.63 8.17 3 1.33 2.83 3.67 4.50 5.17 6.00 7.00 7.80 8.50 9.33 4 2.47 4.00 6.20 7.30 8.33 9.67 10.77 11.83 12.89 13.78 5 4.67 6.50 8.17 9.83 11.30 12.67 13.83 15.08 16.17 17.33 Conclusion of Results On the graph, the potato chip with the largest surface area had the greater gradient. Carol James 10R Biology Coursework ...read more.

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