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To investigate the effect of different concentration of sucrose solution on potato tissue.

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Introduction

Aim: To investigate the effect of different concentration of sucrose solution on potato tissue. Equipment: Sucrose Solution Water 3 x test tubes 3 x measuring cylinders Test tube rack Scales Stop-clock Method: I am going to use the following concentrations of sucrose solution in this experiment: 1. 10ml of water (0M) 2. 7ml of water, 3ml of sucrose (0.25M) 3. 5ml of water, 5ml of sucrose (0.5M) 4. 3ml of water, 7ml of sucrose (0.75M) 5. 10ml of sucrose (1M) 1. Weigh a potato chip 2. Using a measuring cylinder measure out the first of the above solutions. 3. Pour the solution into a test-tube and place in the test-tube rack. 4. Put the potato chip into the test-tube 5. Time 5 mins on the stop-clock 6. Remove the potato chip from the solution 7. Weigh it again 8. Calculate the mass it has gained/lost and record the results 9. Repeat experiment 3 times to ensure fair test 10. Repeat all above steps using all the different solutions described above Variables: The variables I will control will be: The volume ...read more.

Middle

0.07 Water 7ml, Sucrose 3ml (0.25M) Attempt Weight before Weight after Loss/gain 1 1.25 1.29 0.04 2 1.25 1.27 0.02 3 1.25 1.31 0.06 Avg. 0.04 Water 5ml, Sucrose 5ml (0.5M) Attempt Weight before Weight after Loss/gain 1 1.17 1.16 -0.01 2 1.17 1.17 0 3 1.17 1.13 -0.03 Avg. -0.013 Water 3ml, Sucrose 7ml (0.75M) Attempt Weight before Weight after Loss/gain 1 1.3 1.24 -0.06 2 1.3 1.29 -0.01 3 1.3 1.24 -0.06 Avg. -0.043 Sucrose 10ml (1M) Attempt Weight before Weight after Loss/gain 1 1.48 1.43 -0.05 2 1.56 1.39 -0.17 3 1.26 1.14 -0.12 Avg. -0.113 Conclusion: These results show that the weaker the solution, the more water passes into the potato tissue through osmosis. So, the stronger the solution, the more water passes out of the potato tissue through osmosis. This agrees with the prediction I made because I considered the following evidence: These results occurred because the potato membrane is a selectively permeable membrane which only allows water to pass through it. ...read more.

Conclusion

Also I made sure that the controlled variables were kept the same. However, it is difficult to get a clear picture because the change in weight was so small. For example, many changed by just 0.01 of a gram. This means that if the scales were just slightly inaccurate it could have affected the results hugely. Bearing this in mind, if I were to do this experiment again I would leave the potato chips in the solution longer so the change would be more noticeable. Also if I used bigger chips the change would be more noticeable as well. There were no anomalies in my results because, as mentioned above, all the results fitted the genera pattern. To extend this investigation I could see if the size of the potato chip affected the rate of osmosis. I would keep the concentration of the solution the same and just experiment with the size of the potato. If I proved that the size of the potato chip did affect the rate of osmosis I would then perform this experiment again, but making sure each potato chip was of a set weight or size. ...read more.

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