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What effect does the sucrose concentration have on osmosis?

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Osmosis Coursework: What effect does the sucrose concentration have on osmosis? Aim of Investigation: Investigating osmosis in potato chips in relation to the concentration of the solution that the chips are tested in. What is osmosis? Osmosis is the net movement of a liquid across a selectively permeable membrane from a solution/area of a high concentration to a solution with a lower concentration. Osmosis is a type of diffusion that involves a liquid (usually water) rather than other state's (solid or gas) of molecules. So in full, Osmosis is the diffusion of a solvent through a semi-permeable membrane from a dilute to a more concentrated solution. What is sucrose? Sucrose is sugar which is found as a disaccharide naturally in many plants. It is used for production of sugar. Formula of Sucrose: C12H22O11 What is a disaccharide? A disaccharide is a sugar consisting of two linked monosaccharide units. What is a monosaccharide? A monosaccharide is a simple sugar such as glucose or fructose that cannot be broken down into simpler sugars. The effect of sucrose concentration on water and osmosis Water always travels from a high concentration to a low concentration. Due to this fact, when there is a high concentration of sucrose in water, the water particles will diffuse into the sucrose - as sucrose has a high concentration of sugar and a low concentration of water. The effect of water movement on the cell Plant cells always have a strong cell wall surrounding them. When they take up water by osmosis they start to swell, but the cell wall prevents them from bursting. Plant cells become turgid when they are put in dilute solutions. Turgid means swollen and hard. The pressure inside the cell rises; eventually the internal pressure of the cell is so high that no more water can enter the cell. This liquid or hydrostatic pressure works against osmosis. ...read more.


Furthermore, I will repeat the experiment three times and take the average measurement in an attempt to get the most reliable and accurate results possible. Moreover, I rolled the potato in a tissue before weighing after it had been taken from the test tubes. Preliminary Studies As part of my preliminary studies I undertook one experiment of each concentration. I am to try and conclude: 1. If the length 4cm by 1cm by 1cm is good enough. 2. If I will keep the concentrations as they are. 3. If the method should remain the same. Concentration Before mass (g) After mass (g) Difference in mass (g) % difference in mass (2d.p) 2M (20ml of Sucrose) 2.95 1.61 -1.34 -45.42% 1.5M (15ml of Sucrose and 5 ml of Distilled water) 2.76 1.56 -1.2 -43.47% 1M (10ml of Sucrose and 10ml of distilled water) 2.88 1.93 -0.95 -32.98% 0.5M (5ml of Sucrose and 15ml of Distilled Water) 2.91 2.87 -0.04 -1.37% 0M (20ml of Distilled Water) 3.04 3.39 +0.35 +11.51% Implications of Preliminary Studies 1. If the length 4cm by 1cm by 1cm is good enough. 4cm took a long time to cut and the length was quite long - the top of the potato was barely contained in the 20 cm3 solution. From the preliminary I can see that using 4cm would be a mistake. Instead I am going to use 3cm. Each potato will be cut 3cm by 1cm by 1cm and then put in the solution. 2. If I will keep the concentrations as they are. I think that the concentrations used are fine and the results they gave me are comparable and reliable. Making each of the solutions was remarkably easy - as I had all the resources at my hands. 3. If the method should remain the same. I think that the method used was efficient and easy. It was extremely easy to repeat and since I have carried it out so many times I have gotten used to it. ...read more.


This problem could easily be solved if a cork boror is used to cut the potato. To increase accuracy, the experiment could have been carried out even more times. I could also have tested a larger range of concentrations to expand my investigation. Furthermore I could have measured the mass in intervals rather than after 24 hours e.g. every 2 hours and I could have also let the investigation run longer than 24 hours - this way I could possibly find the saturation point. Moreover I could have weighed the potatoes on a more accurate scale e.g. not to 0.00g but to 0.0000g. To further research the field of osmosis I could conduct other investigations exposing the same weight pieces but different surface areas. This way I can see how the surface area of a potato affects the amount of osmosis that takes place. I could use the results obtained from this experiment to compare the differences and similarities of the end results of both investigations. I could also use a larger variety of concentrations; this would make the estimation of concentrations in between those tested in this investigation much more accurate. Another way of investigating osmosis further would be by seeing the effect of concentration on different types of potatoes e.g. red or baked etc. or using entirely different plants (e.g. plants, other vegetables, fruits or a different type of potato). This would test how different semi-permeable membranes could affect osmosis and the movement of water out and into organisms. I could also conduct an investigation into how osmosis is affected when it occurs it different conditions, i.e. how osmosis occurs under a high or low temperature. I could also further investigate this field by seeing how much heat a semi-permeable membrane can withstand before it disrupts. Moreover, I could then do another investigation to see how exposing the potato to heat beforehand affects osmosis. ...read more.

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