What factors affect the activity of enzyme catalyse, on hydrogen peroxide?

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What factors affect the activity of enzyme catalyse, on hydrogen peroxide?

Skill Area P

The problem

The problem that we are going to investigate will be what factors

affect the activity of enzyme catalyse, on hydrogen peroxide. The

factors we should investigate are

1. Temperature

2. Amount of enzyme

3. pH level

4. Amount of substrate (hydrogen peroxide)

Background knowledge

An enzyme exists in all living things. They are produced in all

living cells. In each cell there are several hundred enzymes, which

catalyse at a vast number of chemical reactions. Without enzymes the

reaction would be to slow and life would grind to a halt.

Enzymes dramatically increase the rate at which a reaction

occurs in living things without being used up or effecting the

reaction in any way, which enables it to be used up over and over

again.

An enzyme saves the need to change the temperature, pH level or

concentration to speed up the reaction which could harm the organism.

Enzymes are proteins, which is quite odd as they break and

digest proteins. There is only one type of enzyme which speed up the

breakdown of hydrogen peroxide into oxygen and water in your body.

Active liver cells in mammals produces a poisonous chemical

called hydrogen peroxide, which is very harmful to your liver. It

must be removed if liver cells are not to be harmed.

The enzyme catalyse breaks down hydrogen peroxide into water and

oxygen, which can be disposed of easily.
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I have drawn a diagram on the next page, to show how the enzyme

catalyse breaks down hydrogen peroxide.

1a

[image001.gif]

[image003.jpg]

Prediction

In this experiment I will be changing the amount of enzyme

catalyse in the experiment by adding more potato discs into the test

tube containing hydrogen peroxide.

With my background knowledge, I believe that the rate at which

hydrogen peroxide is broken down into oxygen and water will speed up.

I believe this as enzymes bind with the hydrogen peroxide (the

substrate) and ...

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