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Biology Lan - factors affecting enzyme activity

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Introduction

Factors Affecting the Rate of Enzyme Activity 2008-11-21 DESIGN: Question: How do changes in substrate concentration, enzyme concentration, and environmental factors, such as temperature and pH, affect the rate of enzyme activity? Hypothesis: If the temperature of the catalase increases, then the rate of catalase activity will process faster compared to the temperature of the catalase at room temperature. If the temperature decreases, then the rate of catalase activity will process slower compared to the temperature of the catalase at room temperature. Since the room temperature stays neutral, I think that the rate of enzyme activity for room temperature will be the average between high and low temperature. If the concentration of pH is either more acidic or basic, then the rate of catalase activity will process slower. If the concentration of pH is neutral, then the rate of the catalase activity will process the fastest. If there is an increase in amount in substrate concentration, the catalase activity will process faster. Variables: Controlled variable is time because it doesn't make changes and it can't be controlled. Independent variable is the temperature of the catalase because it doesn't change but other variables change depending on the temperature. Dependent variables are the amount of gases released from the vial with catalase because the amount of gas differs depending on the temperature of the catalase. Materials: - - Prepared catalase (chicken liver extract) - Hydrogen peroxide (3% solution) - Forceps - Trough - Graduated cylinder - beakers (5) ...read more.

Middle

Data Processing: Overview: The raw data which was collected while processing the experiment with factor as temperature were represented in a table format. The assay was to use. Since tables are very useful when presenting two dimensions of information or viewing the data in an easier way, the raw data was presented in a table format. The raw data was also represented in error bar to examine how much error could have been made while processing the experiment. Sample Calculation: Standard Deviation: 1st trial for 5�C Observation (x1) Deviation (X1-) (Deviation)2 (- x1)2 15 +0.6 0.36 17 +2.6 6.76 14 -0.4 0.16 16 +1.6 2.56 10 -4.4 19.36 S2 = ?(- x1)2 n-1 S2= [(15-14.4) 2+(17-14.4) 2+(14-14.4) 2+(16-14.4) 2+(10-14.4) 2] 5-1 = 29.3 4 = 7.3 S = S = 2.70185 T-Test: T = (1-2) = (21-8.6) = 12.4 = 12.4 = 2.152897405 = 2.15 Degree of freedom = 8 T-test = 2.15 Probability value = 0.025 Presentation: Figure 1. The bar graph represents the average of the volume of oxygen evolved for temperature with standard deviation. Figure 2. The bar graph represents the average of the volume of oxygen evolved for pH. Figure 3. The bar graph represents the average of the volume of oxygen evolved for substrate concentration. Figure 4. The bar graph represents the average of the volume of oxygen evolved for catalase. CONCLUSION & EVALUATION: Conclusion: Based on the data obtained from the experiment, my hypothesis was wrong. ...read more.

Conclusion

The agitation of the vial was 90 seconds long and this prevents us from proceeding with the experiment quickly. Another thing which we didn't do very well were the lab planning. Even though we planned ahead of what were going to do, our group changed the lab plan several times. This held back us from continuing the lab and this made us waste our class time to work on the lab. From the error bar, standard of deviation shows that there were quite lots of variation around the mean. The result of the t-test was 2.15 and the probability value was 0.025. This means that differences in data are due to chance 2.5 % of the time. 2.5 % is less than a 5% possibility that the difference between means is due to chance. This means the experiment was quite precise since it had low probability of happening due to chance. Suggestions for Improvement: Some of the suggestion for improvement for this lab is to have wider range in temperature. For this lab, we had five different temperatures to compare the rate of enzyme activity. However, we couldn't find out the rate of enzyme activity at drastic temperature. For next lab, we could get more beakers and set them up in wider range of temperature to see when the rate of enzyme activity stops completely. To find out the rate of enzyme activity at drastic temperature, we can set up the temperature of the water right before the melting point and boiling point. This will help us to find out how the rate of enzyme activity processes at wider range of temperature. ...read more.

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