• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
  12. 12
    12
  13. 13
    13
  14. 14
    14
  15. 15
    15
  16. 16
    16
  17. 17
    17
  18. 18
    18

The effect of toliet cleaning products on E-coli

Extracts from this document...

Introduction

Is the bacteria Escherichia coli (e coli) adversely affected (killed) by King white cleaning products. Higher level biology 14/04/2010 Contents page Page Content 1 Title page 2 Contents page 3 1.1.1- Focus question, 1.1.2- Hypothesis, 1.1.3- Theory 4 1.2.1- Variables , 1.2.2- Control experiment 5 1.3.1- Martials and apparatus, 1.3.2- Safety aspects 6 1.3.2- Safety aspects (cont) 7 1.3.3- Method, 1.3.4- Experimental setup 8 2.1.1- Changes to experimental design 2.1.2- Qualitative data , 2.1.3- Raw data table 9 2.2.1- Overview, 2.2.2- Calculations, 2.2.3- Experimental setup 10 2.3.1- Processed data table, 2.3.2- Process data graph 11 3.1.0- Conclusion 12 3.1.0- Conclusion (cont) 13 3.2.0- Limitations of experimental design 14 3.3.0-Impovements to experimental design 15 Bibliography 1.1.0 Experimental design 1.1.1 Focus question- Is the bacteria Escherichia coli (e coli) adversely affected (killed) by King white cleaning products. 1.1.2 Hypothesis - King White toilet cleaner will most adversely affect (kill) the bacteria Escherichia coli (e coli) to the highest degree when compared to a range of toilet cleaning products. 1.1.3 Theory- Escherichia coli (E coli) are bacteria which causes severe stomach cramps and diarrhoea. It is the leading cause of bloody stools and in older people/younger children certain strains of e coli can be fatal (Family doctor, pg 1, 2008). E coli are common bacteria in humans and some mammals. It is vital in the body as it produces vitamin K as a by-product and is generally a harmless pathogen providing that it remains within the large intestine. However its bi-product becomes negatory when e coli move into other diameters of the body such as the stomach. The bacteria are most commonly transmitted by consuming tainted meat, contaminated water, and raw milk and between cattle workers. However the bacteria may also be transmitted by unhygienic bathroom practise (Pinkster E, pg 1, 2007). E coli is found in human waste which is passed through bathroom toilets, hence some of these e coli bacteria may contaminate bathroom surfaces. ...read more.

Middle

These zones where circular but many where not perfectly circular. Some had diameters that where larger than others. The measurement of diameter was taken from the largest point for all samples. Some zones of inhibitions crossed with other and some even hit the edges of agar plate, like the other samples the largest complete diameter of these particular samples was found. 2.1.3- Raw data table (table 3) - The diameter in millimetres of the zone of inhibition in a e coli growth colony after 24 hours when tested with different cleaning products. Sample Diluted water (mm+0.1) Bleach (mm+0.1) Harpic toilet cleaner (mm+0.1) Home brand toilet cleaner (mm+0.1) Duck toilet cleaner (mm+0.1) King white toilet cleaner (mm+0.1) 1 0 34 15 8 7 20 2 0 33 19 16 10 29 3 0 30 12 11 11 26 4 0 27 17 13 9 34 5 0 25 19 15 13 33 2.2.0 Processing raw data 2.2.1- Overview- Each different cleaning product showed results that were fairly similar. Hence the independent values can be averaged (including the calculation of standard deviation). This average will represent the average diameter of the zone of inhibition for a given substance. These averages with respective standard deviations will be placed into a bar graph so that the effectiveness of different products can be visually compared. 2.2.2- Calculations used (table 4) Statistical Analysis Formulae Sample Calculation Mean X=Mean value X=Sum of all values n= Number of values Mean of bleach diameter (mm+0.1) X=(34+33+30+27+25)+0.1/5+0.1 =29.8mm +0.1 Standard Deviation X -X)2= Variance squared N= Number of values is system /4 (mm) =3.83mm standard deviation 2.2.3- Experimental setup (figure 2)- inserted a bit later 2.3.0 Presenting raw data 2.3.1- Processed data table (table 5) - The average diameter (mm2) of the zone of inhibition of e coli growth colonies after 24 hours for difference different cleaning products. Diluted water Bleach Harpic toilet cleaner Home brand toilet cleaner Duck toilet cleaner King white toilet cleaner Avenge diameter (mm+0.1) ...read more.

Conclusion

Systematic Use better research methods to find how the specific chemical make-ups of different solutions would affect bacteria, or view dead bacteria under a microscope to examine physiological changes. Some samples had higher viscosity then others Systematic Allocate a static volume for each solution and use the volume for each sample. Some samples diffused more than others Systematic Physically spread solutions (dense and thin) over the same surface diameter to reduce the impact of diffusion. Unequal spreading of e coli over agar plates Systematic Use the continuous line cross section grid method to ensure the bacterial colonies are equal in size/density, and have been drawn from the same source. Movement of agar plates Systematic The movements of agar plates should be kept to an absolute minimum. This can be achieved through improved experimental practice. For instance the agar plate should be open, e coli colonies prepared, and then all samples placed within the same time period. Using this method the agar plate only needs to be opened once. Shape of the zone of inhibition Random The number of substances on each agar plate should be reduced or the size of the agar plates should be increased. This will help to ensure the a more accurate circle is produced (does not collide with agar plate edges, ect) Decontamination mechanism was shared amongst others Systematic The decontamination mechanism (methylated sprits) was shared with another experimenter using different solutions. For the purpose of decreasing the opportunity for cross contamination individual decontamination mechanisms should be employed. It may also be responsible to use separate decontamination vessels for individual substances. Timing of recording results Random The recording of results should be taken as per the experimental design. If this time cannot be reach by the experimenter then another or partner experimenter should be requested to record results. Movement in the diameter of the experiments Random Distractions and the movement of other experimenters increase the chance of cross contamination and other random errors. Future experiments should be performed away from other experimenters and a solitary environment. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    Neurology and Behaviour. Focus question: Is there an increase in the perception and ...

    5 star(s)

    However as the variation shows some of the female participants were highly disgusted with the blood, with one rating it five out of five for disgust. (Table Three). Another reason why the results were so varied may be due to the participant's individual personalities or health issues which were not investigated or determined at the time.

  2. Biology Lab Report - Effects of Mouthwash on Oral Bacteria

    Number of filter papers 5. Type of agar 6. Temperature the petri dishes are incubated at 7. Time period for which petri dishes are incubated 8. Size of petri dish Dependent Variables: 1. Size of the zone of inhibition 4. Materials 1. Nutrient agar plates 2.

  1. Biology IA - Investigation of the relationship between surface area and volume using different ...

    Conclusion and evaluation The table 1 indicates that the rate of diffusion into 2% agar cubes does not depend on their size; no matter what was the size of the cube, NaOH travelled 0.5 centimeters per 10 minutes, which means the rate is 0.05cm/min.

  2. The effect on osmosis on the egg shell

    not greater either lesser than 11 (what may indicate not great mass change e.g. because time of experiment was too short) and standard deviation exceeded "1"only in the first set, thus results seem to be relevant. Tables 2-4 present results for each measurement.

  1. biology extended essay - How different diets: vegetarian, vegan and a meat centered diet ...

    Other advantages of vegetarianism and veganism are; * Increased energy. * More food resources and food growing space. * More humane treatment for animals. * Improved quality of life because of lower incidence of some chronic diseases. * Less expensive food sources. * Lower death rates for some chronic diseases.

  2. Biology - Observing the Process of Guttation

    Once again, we are able to see that there are no exceptional anomalies illustrated although these numbers have a slightly larger range as compared to those in Figure 11. For example, this graph shows that on Day 10, the heights of plants E and F have the greatest variation out of any other cases.

  1. In this extended essay I am looking at the effect of different kind of ...

    They are leguminous plants which needs less rainfall. Since they are leguminous plants, that means they have tendency to fix nitrogen in the soil and hence they are known as "nitrogen fixation plants". They are very high in protein. They are also very low in fats. They are very high in dietary fibres and are very good source of zinc.

  2. Testing the effect of substrate concentration on enzymes

    EVALUATION Even though the experiment went along as planed to some extent, it did not really expose all the details I was looking forward to proof from my hypothesis. This was basically because the concentrations used in the experiment were much lower there fore I couldn?t get to the place where it would even out.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work