The aim of this investigation is to make a series of dilutions using the correct liquid handling technique with a pipette, and then finding out the optical density for each of the solutions when they are pipetted into a 96-well plate.

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Liquid Handling

Abstract:

An investigation into the use of handling pipettes and finding out the optical density for each of the dilutions was carried out. This involved using reversed pipetting technique and setting up different sorts of dilutions. It was found that the neat (undiluted) dilution had the highest optical density since the waves were moving more slowly in this solution. An anomaly was found in of the results, suggesting the pipette was used (for that dilution) in an inaccurate way.

Introduction:

The aim of this investigation is to make a series of dilutions using the correct liquid handling technique with a pipette, and then finding out the optical density for each of the solutions when they are pipetted into a 96-well plate.

Single-channel pipettes are used frequently by chemists for measuring various amounts of volumes. When using a pipette the user must make sure that it is very clean whilst used in the laboratory. If the pipette is not clean whilst in use, this could cause various problems such as contaminating the solution thus ending up with error results. Some organisms such as hydrophobic micro-organisms can stick to the plastic tip very readily.