• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzyme studies using acid Phosphate. This experiment will investigate the effect of pH on enzyme activities.

Extracts from this document...

Introduction

Enzyme studies using acid Phosphate Introduction: This experiment will investigate the effect of pH on enzyme activities. Enzymes are biological catalyst, made of protein and held together by peptide bonds. Catalyst accelerates the rate of reaction by lowering the activation energy. The rate of reaction is dependent on several factors which include pH, temperature. Another dependent factor which is worth considering is the product and substrate concentration, which become a rate limiting factor at certain point during the catalytic process. Acid phosphatase is the name given to a general enzyme activity which is present in most eukaryotic cells. This enzyme hydrolyzes a phosphate group from a variety of natural and artificial substrates. The substrate for an acid phosphatase reaction must contain a phosphate functional group attached to another molecule through a chemical bond involving the phosphate and a hydroxyl (-OH) or alcohol functional group. The products of the reaction are the free phosphate group and the rest of the molecule with a free -OH group. ...read more.

Middle

Using a 10ml pipette measure out different amount of standard 200µM p-nitrophenol solution. It is important that all volumes are made up to 6mls with 0.1M of NaOH. The content of the test tubes should mix gently and after mixing, place it in the spectrometer and read at 405nm. After taking all the reading, a table should be drawn and a standard curve. In part B of the experiment, a total of sixteen test tubes is needed. A total of 8 tubes containing 5.5 ml of 0.1M NaOH should be set aside for a later stage of the experiment. Label the remaining 8 test tubes pH 3 to pH 8. Then Add 2.0ml of 2.5 mM p-nitrophenyl phosphate to each tube. At this stage the test tubes should be placed in a water bath for 4 minutes, and to allow it to equilibrate at 37?C. After the stipulated time add 1.0 ml of distilled water to test tube one labelled pH 3 blank, this tube will used as the blank. ...read more.

Conclusion

0.0 0.196 3.82 41.21 40.78 4.54 3.09 8.46 Equation: y = 0.0138x + 0.1473, by using this equation I so able to substitute y which is the absorbance and find the p-nitrophenol concentration which is x. For example: y = 0.0138x + 0.1473 0.15 = 0.0138x + 0.1473 Therefore: x = 0.15 - 0.1473 / 0.0138 x = 0.196 µM Optimum pH = 5.0 (41.21µM) Discussion and Analysis: The experiment went according to the pre-set method, and the overall objectives have been achieved. In term results and data, when compared with other in class data, it seems that the general aims of the experiment have been achieved. A structural change in the enzyme was inevitable as the pH would alter the enzyme shape as well as the substrate. There was not much complication with the experimental methods; the only source of error identified is timing. A delay in the second part of the experiment could affect the results. The optimum pH was the main indicator of success or completion of the experiment; it is observable from the smooth curve graph that the optimum activity has been achieved. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our University Degree Chemistry section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related University Degree Chemistry essays

  1. Synthesis and Isomerization of Nitro and Nitritopentaamminecobalt(III) Chloride, Linkage isomers.

    The spectra of the heated nitropentaamminecobalt(III) chloride also helps prove the purity by being virtually identical to the synthesized product; this means that all of the nitrito starting product isomerized to its nitro form. Nitritopentaamminecobalt(III) chloride also had vibrations that did not appear on nitropentaamminecobalt(III) chloride.

  2. Determining the concentration of copper(II) ions

    The solutions that were made were all made up to 1 dm3 instead of 100cm3 which further ensures a higher degree of accuracy of the results. The calibration graph for this analysis reads that the concentration is 0.005, which when multiplied back (reverse of the dilution), is 0.50 mol dm-3.

  1. Food safety legislation

    EC Regulation 178/2002 3.3 Food Business Operator. In the absence of the owner, manager or chef there should be a trained, competent person to supervise the food safety and hygiene within the premises EC Regulation 178/2002 Article 18 - Traceability All food should be purchased from a nominated supplier, who

  2. Kinetics: The rates of a chemical reaction

    You can then plot the rate as a function of [A]. For a zeroth order reaction, you will get a horizontal line, because rate = k (a horizontal line) rate | / | /rate = k [A] | / |- -/- - - - - rate = k | /

  1. Experiment 4 - Preparation and Reactions of Boric Acid, H3BO3

    and flammable substance is formed. Trimethyl Borate burnt with a green flame without the need to add additional colorants. However, we should be caution as the colour produced by barium and copper ions is also green colour which is very close to our product.

  2. Investigating the Rate of Cooling

    see the temperature dropping more steeply and cooling quicker with the fan as I predicted. This graph show what I expected with the experiment without the fan higher up meaning that at the time points I measured the temperature was higher.

  1. Steam Distillation of (S)-(+)-Carvone from Caraway Seeds and (R)-(- )-Carvone from Spearmint Leaves. Analysis ...

    Applying Raoult's law for immiscible component (X) in a heterogeneous mixture with water, partial pressure of X equals vapor pressure of X. Since X is not soluble in water, it does not depend on its mole fraction in the mixture.

  2. The two central objectives of this experiment are the production of Aspirin from esterification ...

    Expected mass of Aspirin: 0.017391 x 180 = 3.130 (3 decimal places) The solid ASA formed contains impurities and should be recrystallised to achieve a purer state. Recrystallization is possible because most solids are more soluble in hot solvents than in cold solvents.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work