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A colorimetric method for the estimation of glucose in solution

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Introduction

A colorimetric method for the estimation of glucose in solution Aim: To find percentage of glucose in an unknown glucose solution. Apparatus; Unknown glucose solution, 1% glucose solution, colorimeter, cuvettes, water bath, test tube rack, test tube, distilled water. I will use 1% glucose solution to make up the dilution series as follows. Composition Tube % glucose 1% glucose (cm3) distilled water (cm3) 1 ---- 0 10 2 0.1 1 9 3 0.2 2 8 4 0.3 3 7 5 0.4 4 6 6 0.5 5 5 7 0.6 6 4 8 0.7 7 3 9 0.8 8 2 10 0.9 9 1 11 1 10 0 When making up the compositions of glucose, I will use a teat pipette to get the required amount of glucose/water and put them together in the test tube. ...read more.

Middle

The reason that I have used 13 boiling tubes of Benedict?s is so that one can be used for the unknown glucose solution, another as a control for the Benedict's. I will then remove 5cm3 from each of the test tubes of glucose solution, place them in to a boiling tube of Benedict?s reagent and shake the boiling tube. I will label each of the boiling tubes with a pencil. The boiling tubes will then be placed back in to the water bath at 100oC for 10 minutes. Whenever I put a number of test tubes in to a water bath, I will place them in a rack, then place the whole rack in the water bath and on removing, remove the whole rack. ...read more.

Conclusion

I can now place each of the cuvettes in to the colorimeter to receive a % transmission of light. I will repeat this experiment three times, and find an average of the final colorimeter result to reduce the affects of anomalies on my results. I will record my finding in a table as below. % light transmission Solution from tube; Run 1 Run 2 Run 3 Average 1 2 3 4 5 6 7 8 9 10 11 12 (unknown solution) 13 (control of Benedict's) I will then plot a graph including the average results for test tubes 1-11. I can find the composition of the unknown glucose solution by finding where the average results from test tube 13 lie on the line. ...read more.

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