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An Investigation into how temperatures affect the activity of catalyse

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Introduction

An Investigation into how temperatures affect the activity of catalyse Introduction Catalyse is a biological catalyst which can speed up the breakdown of hydrogen peroxide into water and oxygen. You can find the yeast, apples and especially liver catalyse is released in living cells because it is poisonous. Research (Background information- all information on pages 22 + 23 of The Living World) Preliminary Experiment (using 1cm of yeast) Amount of H202 Height of froth (cm) Average height of froth after 15 seconds (cm) Attempt 1 Attempt 2 0.5 1 1.4 1.2 1.0 3.5 3 3.25 1.5 6 5.5 5.75 2.0 6.3 6.4 6.35 2.5 7 7.2 7.1 3.0 9.5 9.4 9.45 Planning The range of temperatures I will use are 20?C, 30?C, 40?C, 50?C and 60?C Appropriate apparatus I will use: * 2 test tubes * Beaker * 2 measuring cylinders * Kettle * Goggles * Pipet * Thermometer * Stop-clock * Hydrogen peroxide * Yeast * Ruler (cm) Method First thing I will do is get 2cm of yeast and 1cm of hydrogen peroxide using a pipet and put the two solutions into two separate test tubes. Then I will boil a kettle and put the hot water into a beaker. ...read more.

Middle

Although enzymes work best at high temperatures there is a cut of point. According to my research enzymes work best up too 40?C/50?C any temperature after this the enzymes denature and slow down causing the height of the froth to decrease. When doing my experiment I have to take into consideration that the heat will escape through the beaker and I will loose about 7?C. So it is possible that the enzymes might work at 50?C Results Temperature (?C) Height of froth (cm) Average height of froth after 15 seconds (cm) Attempt 1 Attempt 2 20 3 3.3 3.15 30 3.4 3.5 3.45 40 3.6 3.4 3.5 50 4 4 4 60 5.9 6.1 6 These results did not entirely work out as I had predicted. When making my prediction I used my scientific research, which said that enzymes work best at around 40?C/50?C but also worked at higher temperature e.g. 60?C as well. Now I am going to do three more temperatures to see whether enzymes carry on working at very high temperatures and see if the height of the froth still increases. Temperature (?C) Height of froth (cm) Average height of froth after 15 seconds (cm) ...read more.

Conclusion

My research shows that most enzymes die and slow down after 40?C/50?C. But my results show that enzymes carry on working up to 60?C. This might have been because the water bath inside the beaker was reading 60?C. But because there is another layer of glass within the beaker the test tube, the hydrogen Peroxide and yeast may be 10?C or so less than what I thought it was actually at. In my opinion my evidence is not entirely good evidence and therefore might not be reliable, some of the results are not in the range of previous results e.g. 60?C. To improve my results if I were to do it again, I would make sure that most of the heat from the beaker could not escape as easy so that it would keep and sustain the specific temperature for longer. Another thing I would change is rather than wait 15 seconds wait longer allowing the two substances to create a bigger or less chemical reaction. The final thing I would change is to have temperature still as a variable but have more temperatures. Not just 20?C, 30?C, 40?C.... but 20?C, 25?C, 30?C, 35?C, 40?C... Although 60?C was much higher than what I had expected my graph near enough matches the graph in my research. Biology Coursework 'Enzymes' Page1 James Baron Candidate Number: 8007 Centre Number: 50451 ...read more.

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