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Analysis of the amino acids in protein by paper chromatography

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Pedro de Carvalho A2 Biology Coursework Analysis of the amino acids in protein by paper chromatography Introduction Amino acids are a group of over a hundred chemicals of which around twenty commonly occur in protein. They always contain a basic group, the amino group (-NH ) and an acid group, the carboxyl group (-COOOH ) as shown in fig. I1 R C H O N C H H OH Fig.I1 All the amino acids have the same basic chemical structure. It is only the R-group that differs. In the amino acid glycine, for example, it is H while in alamine it is CH. ...read more.


The amount of each of the 8 essential amino acids varies in different proteins foods. Some foods have greater value than others. Plants are able to make amino acids by combining some of the molecules produced by photosynthesis with nitrogen obtained from nitrates taken up from the soil. Animals are unable to do this and need to obtain many of the amino acids they require from their diet. From these, they can produce others by the process of transamination. Animals cannot store excess amino acids in their bodies until they are required. The purpose of this experiment is to identify which amino acids are present in the protein present in the egg white. ...read more.


The latter moves slowly up the strip of paper, carrying the amino acids with it. As the amino acids travels at different speeds, they separate from one another. The paper is then treated with a reagent, which stains the amino acids so they can be detected with a reagent, which stains the amino acids so that they can be detected and identified. The advent of chromatography for the separation of amino acids has led to the development of the fully automated amino acid analyser which rapidly produces a qualitative and quantitative print out for any protein material under test. This allows critical assessment of the value of food for human nutrition. ...read more.

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