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Factors Affecting the Action of Copper Sulphate On theActivity of Enzyme (Catalase) Reaction.

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FACTORS AFFECTING THE ACTION OF COPPER SULPHATE ON THE ACTIVITY OF ENZYME (CATALASE) REACTION. AIM The aim of the experiment is to observe the factors that affect the rate of a chemical, the activity of the enzymes and the volume of oxygen produced when an inhibitor is introduced into the reaction. We know that when hydrogen peroxide (substrate) is being catalysed by an enzyme (catalase), it gives product of both oxygen and water. Based on this knowledge we have to determine the effects of temperature, concentration and the PH, as well as how they affect the volume of oxygen produced. 2H2O2 + CATALASE = H2O + O2 PREDICTION (HYPOTHESIS) The substance, which is needed to react with the mixture of hydrogen peroxide and catalase, is copper sulphate (CU2SO) I predict copper sulphate will reduce the rate of reaction between hydrogen peroxide and catalase because it is an inhibitor based on my knowledge. Inhibitors slow down or stop the reaction by competing with the substrate (2H2O2) for the active site of an enzyme (catalase) in due cause slowing down rate of reaction. On the other hand it may act as a cofactor because of the ions which they can produce. Cofactors are non-protein substances, which aid the catalytic action of an enzyme. INTRODUCTION In order to carry out experiment in the investigation, in a successive way, good background knowledge of the rate of chemical reaction occurrence is needed with the factors, that affects it and the order in which they make an effect. ...read more.


When the concentration of enzyme is low and more concentration is added to it, the initial rate of reaction will increase in, when more enzymes is added at that period it is more likely that a substrate will bind to the empty active site on the enzyme. At a higher enzyme concentration, adding more enzymes has no effect on the initial rate of reaction in that the active site of enzyme is fully occupied. SUBSTRATE CONCENTRATION If the amount of enzyme stays the same, the rate of reaction will increase with an increase in substrate concentration, up to a point. When the enzyme active site are all working as quickly as they can, adding more substrate does not have an increase in the rate of reaction. METHOD In this experiment, observations are very much essential, so making observation has to be careful and fast as the reaction is very fast in the experiment. To observe the effects of CUSO4 on the activity of catalase a controlled experiment is needed that is an experiment without CUSO4. It is necessary because it is with this experiment that comparison is made with the experiment that is about to be done. When the experiment with CUSO4 is carried out without a controlled experiment, the effects of CUSO4 won't be known, in that the effect of Catalase on hydrogen peroxide without CUSO4 is unknown and comparison can not be made. ...read more.


The volume should be recorded every 15s. Don't contaminate the substances as it could lead to wrong result and errors. During my trial experiment the range of concentration was very close, making the effect hard to detect. During one of the experiment, the result was anomalous because while we were trying to start the experiment, we used warm water in rinsing the conical flask. This then gave the rise in temperature and increased the rate of collision in the reaction making the result higher. This particular experiment was with copper sulphate, so the result was higher than that we had without copper sulphate. In my next experiment I wish not to repeat that same mistake but to correct it and make my result accurate and valid. CONCLUSION The substance which effect was needed was CUSO4. I can now say it is an inhibitor because during the trial experiment it slowed down the rate of chemical reaction. When the concentration of hydrogen peroxide is higher than that of copper sulphate the rate of reaction is not that badly affected. Temperature I believe has the most powerful effect if we are looking at the factors that affect enzyme activity. It is then followed by the concentration and then it is followed by the pH. Apparatus * 100cm3 gas syringe * 10cm3 measuring cylinder * 3cm3 syringe with needle * 100cm3 flat bottom conical flask * Digital stopwatch * Retort stand * 1 Clamp PROCEEDURE * Arrange all that is needed for the experiment on the table in the laboraratery 1 ...read more.

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