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I am going to investigate respiration in crickets and how temperature varies the rate of respiration.

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Introduction

Planning I am going to investigate respiration in crickets and how temperature varies the rate of respiration. The calculation for aerobic respiration is: Oxygen + Glucose Carbon dioxide + water + energy 6O2 + C6H12O6 6CO2 +6H2O + (J) I will do the experiment safely by making sure that the water will not be too hot or too cold, this is a safety precaution for me as well as the crickets. As we don't want to cause the crickets too much distress or pain. It will be a fair test and accurate because I will: * Use the same crickets * Same mass of crickets * Let the crickets return to room temperature * Reset the measuring device after each experiment * Do each experiment 3 times to get an average I predict that the lower the temperature the lower the rate of respiration. Equipment list: * 2 beakers- a lager one that will hold the different temperatures of water, a smaller one to hold the ink marker. * Crickets- to test on * Bung- containing delivery tube and waste (reset) tube * Ink- to mark distance on the scale * Delivery tube with scale on- to mark distance * CO2 remover- to remove the CO2 * Excess tube for reset. I will measure the amount of oxygen used by the crickets by measuring how far the ink marker has traveled up the scale, the rate of respiration at different temperatures and conditions. ...read more.

Middle

In gray are the anomalous results. In black is the worst result. These experiments that we collected were made fair and accurate by using the same crickets, the same mass of crickets, let the crickets return to room temperature and reset the measuring device after each experiment. Analysis Mathematical analysis of graph Variables Slope 0.1858 � 0.01038 Y-intercept -0.2375 � 0.2171 X-intercept 1.278 1/slope 5.383 Best-fit values SPAN -671.4 K 0.0002780 PLATEAU 671.1 Half Life 2494 Std. Error SPAN 29292 K 0.01234 PLATEAU 29292 95% Confidence Intervals SPAN -75980 to 74639 K -0.03144 to 0.03200 PLATEAU -74640 to 75982 Half Life 21.66 to infinity Goodness of Fit Degrees of Freedom 5 R� 0.9813 Absolute Sum of Squares 0.6892 Sy.x 0.3713 Data Number of X values 8 Number of Y replicates 1 Total number of values 8 I found out that when I decreased the temperature that the crickets were in less oxygen was used up we get this because the ink traveled less at lower temperatures. The Scale had no meaning but the volume inside the tube would mean that less oxygen was used if the ink traveled less. There is a positive linear trend in the results, so the relationship is when temperature is increased the distance of the ink traveled also increased. Conclusion To conclude when you increase the temperature the ink marker travels further up the scale and thus the crickets consume a greater amount of oxygen. ...read more.

Conclusion

If we had gone over this point we would get a curved graph as the distance traveled by the ink would have decreased as the cells couldn't respire properly. So my trend is correct up to that point but after that point the trend doesn't work and a new trend is formed. To improve the experiment I would: * Not use different temperatures of water because of the drowning problem; I'd probably change the temperature of the air around and inside the tube. This would stop the drowning problem * I could use a different insect to see how the rate of respiration varies between insect to insect * I could use a different group of living thing to see how the groups vary. I would have to consider size, mass, amount of cells though. * Warm-blooded and cold-blooded creature see how the temperature would affect their oxygen consumption * Light intensities could cause a difference in respiration rates, as plants can't photosynthesise with any light present. * Change the oxygen and glucose levels These improvements may also help me with my theories and give me a greater amount of evidence to suggest why what happens, happens. They would do this because they would test all aspects of respiration between creatures and so give me a greater amount of evidence. I may find that light is a factor, that cold-blooded and warm-blooded creatures respire differently and so do different groups. By groups I mean: * Mammals * Fish * Plants * Insects * Reptiles * Amphibians Matthew Gooderham 10D Biology coursework ...read more.

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