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Identifying amino acids by chromatography.

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Introduction

Identifying amino acids by chromatography Apparatus * Empty glass coffee jar * Lid for glass jar * Strip of chromatography paper * Capillary tubes * Staples * Oven * Fume cupboard * Ninhydrin solution * Lysine * Leucine * Valine * Glycine * Threomine * X acid * Y acid * Solvent- 4 parts butan-1-01, 1 part ethanoic acid and 1 part distilled water. Method * Fetch and set up equipment. * Pour a small quantity of the solvent into the glass jar. * Cover tank with lid. * On the chromatography paper, make a light pencil mark across the paper 2 cm's from one end. * Using pencil, place the names of the acids on the paper so you can identify them afterwards. * Practice using the capillary tubes on normal filter paper * Using the capillary tubes, place a small amount of each acid on its position along the line on the filter paper. ...read more.

Middle

* When all the solvent has evaporated completely spray the chromatography paper lightly but completely with the ninhydrin solution. * Place the chromatography paper in the oven at 100- 110 degrees for about 10 minutes- or until all the spots have developed. * Take the chromatography paper out the oven. * Circle the spots with pencil. Safety points * Safety goggles must be worn at all times. * Work surface must be clean and tidy before and during the experiment. * Extra care must be taken when using the capillary tubes with the acids. * Any spillage must be cleaned up quickly and safely. * Any breakage must be cleaned up properly. * Do not run in the laboratory, especially when handling equipment. * Take care not to get any of the acids of ninhydrin solution on clothes or skin. ...read more.

Conclusion

I would also take more care in the area I was working in because it was very messy during my experiment and I kept on temporarily losing the capillary tubes etc. I would also take more care in the handling of my chromatography paper because u would have noticed that there is a thumbprint in my results where I have bin handling the paper. Next time I will either use rubber gloves or use tweezers to handle the chromatography paper so my results are not spoilt in any way. I would also take more care when using the capillary tubes because sometimes I made the dots bigger than they should have been. I would also be more aware of what is going around me. This is because the person working next to me spilt one of the acids and I didn't notice and some of the acid got on my chromatography paper. This slightly tarnished the results, as u would have noticed that there are random streaks of purple on my chromatography paper. ...read more.

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