• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Identifying amino acids by chromatography.

Extracts from this document...


Identifying amino acids by chromatography Apparatus * Empty glass coffee jar * Lid for glass jar * Strip of chromatography paper * Capillary tubes * Staples * Oven * Fume cupboard * Ninhydrin solution * Lysine * Leucine * Valine * Glycine * Threomine * X acid * Y acid * Solvent- 4 parts butan-1-01, 1 part ethanoic acid and 1 part distilled water. Method * Fetch and set up equipment. * Pour a small quantity of the solvent into the glass jar. * Cover tank with lid. * On the chromatography paper, make a light pencil mark across the paper 2 cm's from one end. * Using pencil, place the names of the acids on the paper so you can identify them afterwards. * Practice using the capillary tubes on normal filter paper * Using the capillary tubes, place a small amount of each acid on its position along the line on the filter paper. ...read more.


* When all the solvent has evaporated completely spray the chromatography paper lightly but completely with the ninhydrin solution. * Place the chromatography paper in the oven at 100- 110 degrees for about 10 minutes- or until all the spots have developed. * Take the chromatography paper out the oven. * Circle the spots with pencil. Safety points * Safety goggles must be worn at all times. * Work surface must be clean and tidy before and during the experiment. * Extra care must be taken when using the capillary tubes with the acids. * Any spillage must be cleaned up quickly and safely. * Any breakage must be cleaned up properly. * Do not run in the laboratory, especially when handling equipment. * Take care not to get any of the acids of ninhydrin solution on clothes or skin. ...read more.


I would also take more care in the area I was working in because it was very messy during my experiment and I kept on temporarily losing the capillary tubes etc. I would also take more care in the handling of my chromatography paper because u would have noticed that there is a thumbprint in my results where I have bin handling the paper. Next time I will either use rubber gloves or use tweezers to handle the chromatography paper so my results are not spoilt in any way. I would also take more care when using the capillary tubes because sometimes I made the dots bigger than they should have been. I would also be more aware of what is going around me. This is because the person working next to me spilt one of the acids and I didn't notice and some of the acid got on my chromatography paper. This slightly tarnished the results, as u would have noticed that there are random streaks of purple on my chromatography paper. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Physical Chemistry section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Physical Chemistry essays

  1. Objective To illustrate ...

    amino acids, dyes, etc. Its principle is based on the difference of the relative adherence of a solute between a stationary phase and a mobile phase. Since paper consists of cellulose that contains a large number of -OH groups, a layer of water will be permanently attached to the paper.

  2. An Investigation using Chromatography to determine the different Amino Acids

    this is the distance moved by the solvent front. I then measured the distance from each spot of amino acid to the start line, this is the distance moved by each by the spot. I will then work out the Rf Factor for each spot of amino acid and compare

  1. Analysis of amino acids by paper chromatography

    Developing the chromatogram (this was done for us) Dilute Ninhydrin solution in acetone is used to develop the chromatogram. This procedure involves the staining of the amino acids. Ninhydrin is poisonous: It must be used only in a fume cupboard and protective glasses must be worn. Is any of it gets on your skin it must be washed off immediately.

  2. Investigating the Rate of the Reaction between Bromide and Bromate Ions in Acid Solution

    the other results obtained under the same conditions, which are shown below (anomalies are the highlighted results): When I varied the concentration of bromide ions: [Br-] /mol dm3 Repeat 1 Repeat 2 Repeat 3 Repeat 4 Repeat 5 Time/s Temp/K Time/s Temp/K Time/s Temp/K Time/s Temp/K Time/s Temp/K 1.50x10-3 190

  1. Identification of amino acids by using paper chromatography

    With its 4-aminobutyl side-chain, it is classified as a basic amino acid, along with arginine and histidine. It is an essential amino acid, and the human nutritional requirement is 1-1.5 g daily. A deficiency in lysine can result in a deficiency in niacin this can cause the disease pellagra.

  2. Organic Molecule – Lysine.

    It insures the adequate absorption of calcium; helps form collagen that makes up bone cartilage and connective tissues. A deficiency may result in tiredness, nausea, inability to concentrate, dizziness, emotional agitation, bloodshot eyes, retarded growth, decreased immunity, hair loss, anaemia, reproductive problems pneumonia, and acidosis (a pH imbalance in the body).

  1. Identification of amino acids by chromatography.

    This process should then be repeated 40 times, applying the same amount of albumen to the same area, to build up a concentration. This is necessary for the chromatogram results to be clear and distinct. Always remain standing and be careful with the evaporation process, as it is very easy for the chromatography paper to go up in flames.

  2. Column chromatography is a larger scale of thin layer chromatography.

    allow any other molecules of the coloured compound that had bonded with the stationary phase to be attracted back into the solution. Another way is to use a stationary phase which is less polar than the mobile phase. This way, the more polar compound will be attracted highly to the

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work