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Investigate how varying PH affects the activity of the peroxidase enzyme.

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The peroxides investigation. Introduction Peroxides is the incorrect name for this enzyme the correct term is catalase. It catalyses the breakdown of hydrogen peroxide. It is present in all body cells especially LIVER cells which are very active. catalase Hydrogen peroxide water + oxygen catalase 2h2O2 2h2O+O2 Enzymes are biological catalysts and a catalyst is a substance that speeds up the rate of reaction during the reaction it gets changed e.g. If we did not have enzymes we would die because we would not be able to digest the food that we need for proteins and so on. Enzymes get used over and over again, so the body only has a small amount. The reaction can only take place because of the lock and key action the enzymes have a specific shape of the for the thing that it is braking down e.g. there is an active site and if they don't fit together correctly then the enzyme can not do its job to brake down the substance. ...read more.


cylinder will be used each time �The water in the measuring cylinder will be to the top of it each Test �The time the oxygen was collected �The same volume of; Hydrochloric acid Acetic acid Water Sodium carbonate Sodium hydroxide HOW MANY MEASURMENTS WILL BE TAKEN? The amount of measurements taken will be 15,because there is 5 different PH's and I will do them 3 times each so that the results are reliable and accurate. Then they will be averaged. Safety Acids, Alkalis and hydrogen can damage eyesight and irritate skin so goggles will be worn and any splashes on skin will be washed of quickly. Apparatus list �Bowl �Beehive �Measuring cylinder �Tube with a bung on one end �Small bottle cap �Conical flask �Safety goggles �Stop watch. Prediction I predict that the PH7 will work the best because it is neutral and this enzyme works best at PH7 the same shape as the. ...read more.


I predicted that the PH7 would work the best because it is neutral. I also know that the results are correct because I got some anomalies results but the second time I tried them they all looked reasonably similar and they had the same pattern. There are a few things that went wrong during the experiment such as when you had put the liver in and the PH in then when you added the Hydrogen peroxide, you could not get the bung in quick enough because a lot of gas escapes out of the conical flask, so I thought of a way of solving the problem. Also the liver had a few lumps in so it might not worked as well because the liver can not get broken down as quick when it is in big lumps because it decreases the surface area so if the liver was liquidised longer then it would not be as lumpy. ...read more.

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