• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating The Effect Of Temperature Change On The Permeability Of Beetroot Cells.

Extracts from this document...


Investigating The Effect Of Temperature Change On The Permeability Of Beetroot Cells. Aim This investigation aims to determine how an increase in the surrounding temperature affects plasma membrane of a typical plant cell structure. Hypothesis Increasing the temperature above a certain extent will damage and denature the plasma membrane and cause the cytoplasm and other substances contained within the membrane to leak out. Introduction Beetroot has a red pigment (anthocyanin) stored within its cells; it is prevented from leaking out by its cell membrane. The membrane is an extremely thin layer (8 to 10 nanometers (nm)) thick which is partially permeable. As we know, cell membranes are made up of lipids and proteins, and proteins tend to be affected by temperature. This investigation intends upon finding out if temperature has any effects on the permeability of beetroot cells, my prediction is that it does. This is because when the temperature is increased, a number of bonds in the protein molecules of the cell membrane are weakened. ...read more.


* A Beaker * Thermometer * Bunsen Burner, Fireproof Mat, Gauze, stand * A Tile, Scalpel * Test tube rack * Pipette * Beetroot cores cut into discs of approx. 1mm thickness * Cuvettes (Colorimeter) * Measuring tube Plan 1) Cut the Beetroot cores into discs (1mm thickness approx). Try making sure as far as possible that they are all of the same diameter and thickness, so as to reduce any errors unrelated to the actual aim of the experiment. 2) The slicing of Beetroot into discs will cause cells to rupture and release the red pigment, these damaged cells are insignificant and hence make sure to wash the Beetroot discs out in cold-water until they no longer change colour and the water remains clear, thus no pigment is showing. 3) As the pilot investigation seems to indicate the temperature of leakage to be between 40o C - 50o C, set the water bath(s) using conventional apparatus such as Bunsen and tripod etc... ...read more.


9) Move the Cuvette changeover control (located on the left hand side panel) to the "ref " position and, using the coarse and fine sensitivity controls, set the metre to read 100% transmission (or 0% absorption). 10) Move the Cuvette change over control to the test position and read the percentage transmission or absorbance of the standard solution directly from the meter. 11) Make a note of the readings and then do the same for test tubes B-F. 12) After results have been collected, tabulate the results, draw graph and deduce any conclusions obtained from the results. As you can see from my plan, the dependent variable is the calorimeter measurements and the independent variable is the temperature control. Thus make sure all other variables such as volume of water in test tubes, size of discs and calorimeter settings are all kept constant, so as to reduce any inconsistent outcomes. ?? ?? ?? ?? Luqman Patel A/S Biology ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Here's what a teacher thought of this essay

** Plan only included. This plan includes a fairly detailed method but is lacking in a number of key elements that would be expected for A level coursework.
To improve
The inclusion of background research beyond that found in most A level textbooks is expected to help inform the plan. References should be included.
The aim/ hypothesis needs to be clearly stated in terms of the dependent and independent variables.
There should be a full consideration of the key experimental variables and how they are to be controlled. The apparatus that is to be used should be justified as well as listed.
There is no attempt to assess safety and this is usually expected in newer mark schemes.
The method mentions a pilot experiment. If this has been carried out the results should be included and discussed.

Marked by teacher Stevie Fleming 26/07/2013

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    The Effect of pH on Pectinase

    3 star(s)

    x 100/24ml? Percentage Error = 3.75% It is clear that in a classroom experiment the results taken are not as precise as those that can be recorded in the computerized laboratories of top biological research companies. However, this does not mean the results that are taken using the equipment provided are not accurate, and do not show trends and patterns.

  2. The Effect of Concentration on Pectinase Using Apple

    The tables for each concentration and the first graph clearly show that a greater volume of juice is produced in the first five minutes at a higher concentration of pectinase. The average volume produced after 5 minutes using 5.00% pectinase was 2.93ml, whereas the equivalent result for 0.25% pectinase was 1.20ml.


    the same, or 67�C is 1% lower in light transmission than 54�C. This is because once the cell membrane of the beetroot is broken; no difference is made on how much pigment is released. If I had done another temperature preferably at a temperature above 67�C, I would have gotten

  2. Investigating how different concentrations of a antibiotic effects the growth of a bacterium.

    These include: o Sterilisation of equipment and area-during the experiment there where a number of times where the equipment and areas would need to be cleaned and sterilised.

  1. Investigating the effect of temperature on the activity of free and immobilised enzymes.

    to using room temperature milk when it was added to the higher temperatures it cooled them down so the experiment did not happen at the correct temperature. To combat this I will also heat up the milk in the water bath so that temperature stays as close to the chosen temperature as possible.

  2. Permeability of Cell Membrane in Beetroot Cells

    * Thermometers * Cutting tile * Freezing salts/ice * Pipettes * Heat proof mats * Tripod gauzes * Size 5 borer * Glass stirring rods * Matches * Splint * Tweezers Actual Practical: Method: 1. Set up two beakers with 250ml of water at 10?C using ice and freezing salts


    FIGURE 3: A PLASMOLYSED ONION CELL IN A MICROSCOPE X400 3 INCIPIENT PLASMOLYSIS Is the stage just before that, when the cell is just about to become plasmolysed. At this point, the pressure potential is zero. Incipient plasmolysis is defined as the point at which the plasma membrane has just

  2. To investigate one of the factors that affects the permeability of cell membranes.

    lipids in the cell membrane in a similar way to that of alcohol. This means that they also increase the permeability of the cell membranes and cause more of the pigment to leak out in the same way that alcohol does.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work