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The effect of temperature on hatching effect of brine shrimps

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The effect of temperature on hatching effect of brine shrimps Aim: This experiment is being performed in order to determine the optimum conditions for brine shrimp hatching and the conditions that are less favourable for hatching and growth. Hypothesis: Brine shrimp are a species of aquatic crustaceans. Their cysts can remain dormant for several years and are metabolically inactive. This means that they can remain in dry, oxygen free environments for long periods of time without being affected. This is called cryptobiosis. Once the cysts are in contact with salt water, they hatch within a few hours and are around 500 micrometers long upon hatching. During maturity, they can grow to as much as 10mm and have a life span of around 1 year. It is believed that the higher temperatures are more preferential for the hatching of the cysts. This temperature ranges up to around 35�C at which the shrimps find it increasingly difficult to sustain life. In this experiment the highest temperature being used is 30�C, so the shrimps should be able to survive and thrive. This higher temperature is what I believe to be the most suitable temperature for the shrimps. ...read more.


Any excess cysts must be returned to their container for use in the future as they deserve the chance to develop at some stage. Developed shrimps must be disposed of appropriately after the experiment by being placed into a pond or other saltwater environment. Apparatus: * 5 small beakers * 1 large beaker * 3x water baths set at 20, 25 and 30�C respectively. * Fridge set at 4�C * Thermostat controlled biology laboratory at 17�C * Pipette * Brine water (salt solution) * Brine shrimp cysts (40 for each test beaker) * Forceps * White paper, graph paper. * Marker pen (water resistant) * Magnifying lens. Method: 1. Place 2g of sea salt into a beaker for the salt solution. Add 100cm3 of de-chlorinated water and stir. 2. Label the beakers with the conditions they are going to be exposed to. 3. Place some cysts onto a piece of plain white paper. 4. Wet some graph paper that has been cut to a smaller size and count 40 cysts onto this graph paper using forceps. 5. Place the graph paper into the salt water and leave for 20 seconds. ...read more.


There was also an issue of the salt leaving the solution and forming salt crystals at the base of the beaker in the 4�C experiments. this affected salinity by reducing the salt content in solution. This was evident in all trials with cling film over the beakers, but not to the extent of the 4�C experiments which may have contributed to the increased volume of shrimp hatching. During trial number 3 for the 35�C experiment, there was an incident concerning the water bath being turned up to a higher temperature. This essentially killed the shrimp off and resulted in none surviving for the whole week. There was another incident involving the shrimp being knocked over inside the water bath resulting in void results as no shrimp remained in the beaker. This occurred on the trial 4, 17�C experiment. In order to prevent this from occurring in any future experiments, the water bath should contain a grid which can hold the beakers in place and prevent them from floating around. The water bath adjustment is easy to make and is unavoidable if left in a biology lab with students coming in and out all day. There is also no way of preventing the salt leaving the solution in the colder temperatures, without warming the solution up, affecting the results. ...read more.

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