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To find out how is enzymes affect by different type of temperature and see the changes in the colour of the mixture of iodine, starch and amylase.

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Introduction

Aim: To find out how is enzymes affect by different type of temperature and see the changes in the colour of the mixture of iodine, starch and amylase. Prediction: We are going to be looking at temperature and I think 10c will have the all the same colour and the highest will have all different colour. Hotter the water better reaction in the colour changing and colder the water less reaction in the colour changing. As the temperature increases the rate when starch is broken down will increase and lower the temperature the more time it will take to break down the enzymes. Hypothesis: As the temperature increases, the starch is broken down more quickly because the enzymes and starch molecules move more quickly and collide more often. This means that amylase and starch molecules will come into contact with each other more often to react. However, as the temperature increases further, the amylase molecules start to change shape as they get damaged. This means that the starch molecules cannot fit on to the amylase so well and will therefore not react so quickly. ...read more.

Middle

Spotting tile * Thermometer * Pipettes * Stirring rod * Stop-clock * Goggles * Starch solution * Amylase * Iodine solution * Ice Diagram: Results: 1. I will observe colour because to see the different colour the different temperature will have when they are mixed with the solution 2. I will observe the time taken because to see how long does it take to change colour in each solution 3. I will observe the temperature of the water because to see the different reaction in all solutions like 10c or 50c.10c will be cold water and 50c will be hot water both of them will have different reaction. Range: I need to take about 5 temperatures in total and the ratio must be same in all temperature if it's not the same it wont be a fair test and the colour will come out different Repeats: If I repeat some of them I will repeat the lowest and the highest because to make it sure that all of them come out right or I can repeat all of them but it will take time to repeat all of them. ...read more.

Conclusion

Colour 10c 80 Sec Black 20c 70sec Black 30c 60sec Black/blue/yellow 40c 50sec Blue/yellow 50c 40sec Yellow 100c 30sec Yellow/orange Temperature Of Water Colour Of Mixture (Time in Minutes) 10 20 30 40 50 60 70 80 10� 20� 30� 40� 50� 100� Conclusion The results tell us this that there are no changes seen in the colour when the temperature is at the lowest point. But when the temperature is at a higher point, a change in the colour can be noticed. The higher the temperature (100�c) the quicker the reactions and the lower the temperature (10�c) the slower the reactions and most of the changes will happen on the highest temperature (100�c). Evaluation I think I did some temperature wrong because I did not do all of them; I did what I could do. I could have improved my experiment if I did it a bit quicker and if I did concrete more about the experiment and I think my prediction was right and I used all the apparatus properly example wore glosses and the method was a bit messed but still I did what I could do and next time if I did the experiment again I would change the temperature and the ratio of the solution. ...read more.

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