With heat kinetic energy is created which gives the particles in the water more energy so therefore they have a higher chance of cliding and reacting with each other. As results this the starch will break down faster
Science knowledge: As the temperature rises the enzyme works much quicker, but if it gets too hot, above 45ºC the enzymes will denature. The graph below shows this very clearly:
Enzymes are affected by changes in and alkaline conditions. Some enzymes like to work in an acid condition while others in an alkaline condition. Stomach enzymes work best in an acid condition, this is provided by the hydrochloric acid found there.
Variables:
I think I will change the temperature of the mixture, I will change the ratio of the starch and amylase and the time when the solution of starch and amylase mix’s with iodine. Put more starch and more amylase in the solution
Equipment
- Test Tubes
- Baker, 250ml
- Measuring cylinder, 10ml
- Spotting tile
- Thermometer
- Pipettes
- Stirring rod
- Stop-clock
- Goggles
- Starch solution
- Amylase
- Iodine solution
- Ice
Diagram:
Results:
- I will observe colour because to see the different colour the different temperature will have when they are mixed with the solution
- I will observe the time taken because to see how long does it take to change colour in each solution
- I will observe the temperature of the water because to see the different reaction in all solutions like 10c or 50c.10c will be cold water and 50c will be hot water both of them will have different reaction.
Range: I need to take about 5 temperatures in total and the ratio must be same in all temperature if it’s not the same it wont be a fair test and the colour will come out different
Repeats: If I repeat some of them I will repeat the lowest and the highest because to make it sure that all of them come out right or I can repeat all of them but it will take time to repeat all of them.
Safety: We need to make this experiment safe because this could damage your eyes so we need glosses and not play around with iodine people with sensitive eyes might damage there eyes.
Method:
- Measure 15ml of starch solution into a test tube.
- Measure 2ml of amylase into a second test tube.
- Place both test tubes into a water bath at the temperature u want
- Leave for few minutes and record this temperature.
- While the test tubes are in the water bath.
- Place one drop of iodine solution into each dimple of spotting tile.
- With the stirring rod, take a one-drop sample of starch solution from the test tube and place in the first dimple, to show the presence of starch.
- Add the amylase solution to the starch solution and start the stop clock.
- Take a one-drop sample every 30 seconds and test for the presence of starch.
- Record the time taken for the starch to disappear.
- Repeat the experiment at room temperature.
- Do the experiment again at 10c, 20c, 50c
Readings:
Conclusion
The results tell us this that there are no changes seen in the colour when the temperature is at the lowest point.
But when the temperature is at a higher point, a change in the colour can be noticed. The higher the temperature (100ºc) the quicker the reactions and the lower the temperature (10ºc) the slower the reactions and most of the changes will happen on the highest temperature (100ºc).
Evaluation
I think I did some temperature wrong because I did not do all of them; I did what I could do. I could have improved my experiment if I did it a bit quicker and if I did concrete more about the experiment and I think my prediction was right and I used all the apparatus properly example wore glosses and the method was a bit messed but still I did what I could do and next time if I did the experiment again I would change the temperature and the ratio of the solution.