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Vitamin C concentration

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Introduction

Which type of fruit juice provides the most vitamin C? The quantity of vitamin C in food and drink can be determined using a simple colour test. Vitamin C decolourises the blue dye DCPIP (dichlorophenolindolphenol). Vitamin C is an antioxidant and reduces the DCPIP. DCPIP changes from blue to colourless (or slightly pink) as it becomes reduced. We will be testing a range of different fruit juices to see which contains the highest concentration of vitamin C I predict that the fruit juice with the highest concentration of vitamin C will be orange juice, as it is the most widely publicized as having a high vitamin C content. Method Apparatus; Range of fruit and/or fruit juices Standard 1% vitamin C solution DCPIP 0.1% Pipette, syringe or burette to measure volumes accurately Standard laboratory glassware and apparatus To begin with, 1cm� of DCPIP each fruit juice will be measured into separate test tubes and placed in a test tube rack, a burette is then placed in a clamp and filled with each fruit juice. ...read more.

Middle

Safety goggles Breaking glass 2 4 8 Take care Spilling chemicals on clothing 1 5 5 Science overalls Results My results Repeat 1 (cm�) Repeat 2 (cm�) Repeat 3 (cm�) Repeat 4 (cm�) Average (cm�) 1% vitamin C solution 0.50 0.50 1.00 0.50 0.50 Orange 0.40 0.40 0.60 0.30 0.36 Apple 0.70 0.20 0.50 0.40 0.45 Grapefruit 0.10 0.20 0.20 0.20 0.175 We pooled together all the results from each group to get a better data range, the below table includes the data from each group's experiment. As you can see, the trend in the data of the entire group is much the same as ours, grapefruit having the least amount of juice required to decolourise the DCPIP and vitamin C solution having the most. There were a number of outliers in the results, most notably the first apple juice result, this was most probably due to human error in titrating the juice Group Averages Group 1 (cm�) Group 2 (cm�) Group 3 (cm�) Group 4 (cm�) Average (cm�) 1% vitamin C solution 0.67 0.83 0.50 0.50 0.625 Orange 0.53 0.43 0.36 0.56 0.47 Apple 1.10 0.30 0.45 0.33 0.36 Grapefruit 0.37 0.23 ...read more.

Conclusion

= 6.25mg of Vitamin C to decolourise the DCPIP solution. There is exactly 6.25mg of vit c in the volume of each fruit juice put in, i.e in the 0.45cm3 of apple juice mixed with DCPIP there is 6.25mg of Vitamin C. I must then work out the concentration of vitamin c in each fruit juice to then compare them with each other. Concentration (mg/cm3)= amount (6.25)/ volume. Fruit Juice Volume (cm3) Concentration (mg/cm3) 1% Vitamin C solution 0.625 10.00 Apple Juice 0.36 17.36 Orange Juice 0.47 13.80 Grapefruit Juice 0.31 35.71 The most concentrated fruit juice in mg/cm3 is clearly the grapefruit juice. The experiment has produced overall a reliable set of results as most drinks cartons tell us that grapefruit is high in vitamin c and our results help prove this. I do not think that any more accurate apparatus could have been used in this, as far as accurate equipment goes, a burette is the best. I do not think that any extraneous factors were large enough to affect the results to a substantial degree so I believe the data to be valid and reliable as we controlled most things that may have affected it. ?? ?? ?? ?? Taylor Hayes VPr Biology- Unit 1 Core Practical Two ...read more.

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