• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

What Affects The Action Of Catalase Enzyme On The Decomposition Of H2O2?

Extracts from this document...

Introduction

Sadie Tulley Biology Coursework, Sept 2001 Page 1 What Affects The Action Of Catalase Enzyme On The Decomposition Of H2O2? Planning Catalase is a biological catalyst that breaks down Hydrogen Peroxide (H2O2) into Water (H2O) and Oxygen (O2). Below is the reaction that takes place: - Hydrogen Peroxide � Oxygen + Water H2O2 � O2 + 2H2O Reactions can be speeded up through the use of a Catalyst, this is a substance which changes the rate of a reaction but is not actually used up so they can be re-used; in this case we will use Catalase. Catalase is an enzyme, which is found in living things such as yeast and liver, the enzyme is used in the cells to remove Hydrogen Peroxide which is a waste product made by all cells, H2O2 is very toxic and so breaking it down quickly is very important. How does Catalase work? All enzymes work in the same way, there are two main molecules in the reaction, the enzyme and the substrate, the substrate is the molecule the enzyme acts upon. The enzyme has a small section called the 'active site', which is the exact shape to fit around the substrate molecule; this is called 'the lock and key hypothesis'. ...read more.

Middle

All the chemicals I will use will be kept at room temperature before and during the test, this means that they will all be the same temperature (approx. 21�C). To keep the concentration of Catalase the same throughout the test I will sort through all the yeast beads I make so that the ones I use are all the same size and shape, I will also discard immediately any "floaters" as these beads already contain air and so are useless. Instructions 1. Wearing safety goggles take 5cm� of Sodium Alginate Jelly and 5cm� of Yeast Suspension and mix together in a small beaker. 2. Fill up another small beaker with 50cm� of Calcium Chloride. 3. Use the syringe to suck up the jelly and yeast mixture, slowly drop it into the CaCl2 drop by drop, as the mixture goes in it will form small beads, make about 30 beads. 4. The beads should sink immediately to the bottom of the beaker, any that do not should be removed also any that have "tails" or are not round should be removed to keep the test fair. 5. ...read more.

Conclusion

7 predicted would happen, if I was to re-do the experiment I would test a higher concentration of Hydrogen Peroxide to back up this part of my prediction. If I were to repeat the experiment I would probably drop more yeast beads into each concentration of H2O2 so my average would be more statistically valid. The results I have gained may be slightly erroneous because my reaction speed is not very fast, this means that I could have stopped the stop clock too soon or too late making my results inaccurate, to make up for this I rounded the results up to the nearest half a second. Temperature probably did not affect my experiment because all of the ingredients were kept in the same place at room temperature and all the tests were done on the same day within an hour. I could measure the rate of reaction in a completely different way to gain more accurate results; the method I would use to do this would be to use a conical flask to do the experiment in and then catch the oxygen gas produced in a gas syringe, using this method it is easier to read the results accurately, leaving less room for any imprecision. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our AS and A Level Molecules & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related AS and A Level Molecules & Cells essays

  1. Marked by a teacher

    Enzymes - investigate how the substrate concentration (H2O2) affects the activity of catalase on ...

    3 star(s)

    Moreover, when they do collide, they do so with more energy. This makes it easier for bonds to be broken so that the reaction can occur. As temperature continues to increase, the speed of movement of the substrate and enzyme molecules also continues to increase.

  2. Investigating the effects of Copper Sulphate on the action of Catalase Enzyme breaking down ...

    which, along with the hydrogen peroxide molecules, will also compete for the active site of the catalase enzymes. If this happens then it is clear that less hydrogen peroxide will break down during the reaction, as the enzymes will also be breaking down the copper sulphate.

  1. A Level Biology revision notes

    invading bacteria in the lungs * But the inflammation causes damage to the surrounding cells * Lesions may become hard or spongy, leaving "holes" in the lungs * Treated with a cocktail of antibiotics for 6 month Ability to Cause Disease * Depends on o Location - what tissue is

  2. effect of different concentration of H2O2

    Ph level - this is where you would test the same amount of substrate and enzyme but change the ph level. You would probably use a wide range of ph levels to see how it behaves in an acid, neutral and an alkaline solution.

  1. This is an experiment to examine how the concentration of the substrate Hydrogen Peroxide ...

    of it is in the DNA which is individual for each species. Therefore, expect that the catalase from different plants and animals will have slightly different characteristics. Catalase is extremely important in cells because it prevents the accumulation of Hydrogen Peroxide.

  2. Biology coursework

    I had to take an average for each bead size according to the nozzle they came from. In order to do this, I used a micrometer to measure 10 different beads. I recorded these sizes in a table and later took the average sizes according to which nozzles the beads came from.

  1. the effect of catalase concentration on the breakdown rate of h2o2

    When the substrate molecules crash into the enzymes' active site, the R groups of the amino acids, which line the surface of the active site, form temporary bonds with the substrate, creating a momentary enzyme-substrate complex. Whilst in this complex, bonds are either broken down, in a single substrate molecule, or urged to synthesise, between two substrate molecules.

  2. Examine how the concentration of the temperature Hydrogen Peroxide (H2O2) affects the rate of ...

    For example, used to in pesticide for cats and dogs is a kind of yeast inhibitor (acetyl cholinesterase inhibitor). If human or animal suck it into body. It can make people or animal become very irascible. ii) Temperature: As temperature increases, molecules move faster (kinetic theory).

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work