• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of HydrogenPeroxide Concentration On Catalase

Extracts from this document...

Introduction

INVESTIGATING THE EFFECT OF HYDROGEN PEROXIDE CONCENTRATION ON CATALASE ANALYSIS TABLE SHOWING THE AMOUNT OF OXYGEN EVOLVED AT THE DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE. Concentration of Hydrogen Peroxide(M) Volume of Oxygen evolved(ml) Average Concentration of Oxygen Evolved (ml) 1.00 65.0 65.0 63.0 64.3 0.80 53.0 51.0 51.0 52.0 0.60 38.0 38.0 39.0 38.3 0.40 28.0 28.0 27.0 28.0 0.20 15.0 14.0 14.0 14.3 0.00 3.00 4.00 3.00 3.30 Calculating the Rate the Reaction. Rate (ml/s) =Volume of oxygen released (ml) Time(s) TABLE SHOWING THE RATE OF REACTION AT DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE Concentration Of Hydrogen Peroxide(M) Average Concentration of Oxygen Evolved Time (s) Rate (ml) 1.00 64.3 37.2 1.73 0.80 52.0 40.0 1.23 0.60 38.3 42.4 0.90 0.40 28.0 49.3 0.57 0.20 14.3 51.0 0.28 0.00 3.30 59.5 0.55 From the results, I have noticed that the volume of the products (Oxygen) ...read more.

Middle

Because in this experiment I used a constant enzyme concentration, decrease in substrate concentration results in less or limited number of enzyme- substrate complexes as the enzyme's active sites are not working to full capacity, this is because there is less chance of enzymes colliding with the substrate molecules. Which is why there is decrease in the volume of the products and the rate of reaction in the 0.00M concentration of Hydrogen Peroxide? Substrate concentration is limiting the rate of this reaction. However, if the substrate concentration was increased to lets say; 2M, I think the graph of "Average Volume Against Hydrogen Concentration" would tail off when it reaches the maximum point called the" V-max ".The reaction would then become constant because the active sites of the enzymes would gradually become fully utilised and adding more substrate would have no effect on the rate of the reaction. ...read more.

Conclusion

So it has % Error = 1 X 100=1% 100 2. Difficulties in reading the highest volume reached by the froth. The meniscus of the froth was not clear. 3. Inadequate washing of the measuring cylinder-some Hydrogen Peroxide may have remained in the measuring cylinder The above errors can be improved by:- 1. Replacing the measuring cylinder with a more accurate apparatus e.g. Burette. 2. Using the method of displacement to collect the Oxygen gas. It has a good and more accurate way of taking the volume of the oxygen because there is no froth. 3. Use of different measuring cylinder for each dilution, this will lessen the errors because there would be no need to rewash the same measuring cylinder. I don't think the test is reliable because there is too many minor errors which have a great effect on the results. I suggest the use of a manometer would be more reliable and would generally improve the test. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    This is because the higher the concentration of bile the greater the chance of bile colliding with the triglycerides therefore increasing the surface area of the lipids and also the rate in which they are emulsified, and in turn increasing the rate of which the lipase reacts on the larger

  2. The effect of aspirin on the action of bovine liver catalase

    in rate of reaction is -0.10, the percentage difference is -6%, so a variability of � 4.6% in the results for 20gdm-3 means that these results cannot be used to prove that there is a difference in rate of reaction between aspirin concentrations of 15gdm-3 and 20gdm-3.

  1. Effect Of Substrate Concentration On The Activity Of Catalase

    (�C) 10 20 30 40 50 60 70 0 0 0 0 0 0 0 0 30 0 1 2 3 2 1 0.5 60 0.5 2 2.5 3.5 2.5 1.5 0.5 90 1 3 4 4 3 2 1 120 1.5 4.5 5.5 5 4.5 2 1 150

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    However; if somehow the conical flask is shaken accidentally, the bubble would become free to float at the top of the burette. Therefore, the rate of reaction is then influenced. I also noticed that he slower the bubbles are formed, the slower the rate of reaction.

  1. The effect of concentration on the activity of catalase.

    This explains why enzymes are specific to their reactions. It is now found that in some enzymes, the active site changes when the substrate attaches to it. This is for a more accurate fit. This is called the induced fit. When the active site and the substrate join, it forms an enzyme-substrate complex.

  2. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    The larger the surface area of a substance, the faster the rate of reaction. This is because there are more particles per cm3. Therefore, there will be more collisions every second between the substrate molecules and the enzymes. Volume is another key factor that can influences the rate of the reaction between hydrogen peroxide and catalyse.

  1. Study the reaction of Catalase and Hydrogen Peroxide. I am specifically focusing on the ...

    This means it can be reactivated if the pH rises or falls again. However, if the pH rises or falls to the extremes the enzyme may be denatured, meaning it cannot be resurrected. Input and Output Variables In my experiment I am going to investigate the affect changing the concentration

  2. Investigating the effect of enzyme concentration on the rate of an enzyme catalysed reaction.

    The temperature affects the rate of reaction. If you increase the temperature you increase the amount of energy, therefore more enzyme and substrate molecules will collide more often, increasing random movement. The more collisions there are the higher the chance is that a substrate molecule will fit an active fit.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work