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Investigating the Effect of HydrogenPeroxide Concentration On Catalase

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Introduction

INVESTIGATING THE EFFECT OF HYDROGEN PEROXIDE CONCENTRATION ON CATALASE ANALYSIS TABLE SHOWING THE AMOUNT OF OXYGEN EVOLVED AT THE DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE. Concentration of Hydrogen Peroxide(M) Volume of Oxygen evolved(ml) Average Concentration of Oxygen Evolved (ml) 1.00 65.0 65.0 63.0 64.3 0.80 53.0 51.0 51.0 52.0 0.60 38.0 38.0 39.0 38.3 0.40 28.0 28.0 27.0 28.0 0.20 15.0 14.0 14.0 14.3 0.00 3.00 4.00 3.00 3.30 Calculating the Rate the Reaction. Rate (ml/s) =Volume of oxygen released (ml) Time(s) TABLE SHOWING THE RATE OF REACTION AT DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE Concentration Of Hydrogen Peroxide(M) Average Concentration of Oxygen Evolved Time (s) Rate (ml) 1.00 64.3 37.2 1.73 0.80 52.0 40.0 1.23 0.60 38.3 42.4 0.90 0.40 28.0 49.3 0.57 0.20 14.3 51.0 0.28 0.00 3.30 59.5 0.55 From the results, I have noticed that the volume of the products (Oxygen) ...read more.

Middle

Because in this experiment I used a constant enzyme concentration, decrease in substrate concentration results in less or limited number of enzyme- substrate complexes as the enzyme's active sites are not working to full capacity, this is because there is less chance of enzymes colliding with the substrate molecules. Which is why there is decrease in the volume of the products and the rate of reaction in the 0.00M concentration of Hydrogen Peroxide? Substrate concentration is limiting the rate of this reaction. However, if the substrate concentration was increased to lets say; 2M, I think the graph of "Average Volume Against Hydrogen Concentration" would tail off when it reaches the maximum point called the" V-max ".The reaction would then become constant because the active sites of the enzymes would gradually become fully utilised and adding more substrate would have no effect on the rate of the reaction. ...read more.

Conclusion

So it has % Error = 1 X 100=1% 100 2. Difficulties in reading the highest volume reached by the froth. The meniscus of the froth was not clear. 3. Inadequate washing of the measuring cylinder-some Hydrogen Peroxide may have remained in the measuring cylinder The above errors can be improved by:- 1. Replacing the measuring cylinder with a more accurate apparatus e.g. Burette. 2. Using the method of displacement to collect the Oxygen gas. It has a good and more accurate way of taking the volume of the oxygen because there is no froth. 3. Use of different measuring cylinder for each dilution, this will lessen the errors because there would be no need to rewash the same measuring cylinder. I don't think the test is reliable because there is too many minor errors which have a great effect on the results. I suggest the use of a manometer would be more reliable and would generally improve the test. ...read more.

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