• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of HydrogenPeroxide Concentration On Catalase

Extracts from this document...


INVESTIGATING THE EFFECT OF HYDROGEN PEROXIDE CONCENTRATION ON CATALASE ANALYSIS TABLE SHOWING THE AMOUNT OF OXYGEN EVOLVED AT THE DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE. Concentration of Hydrogen Peroxide(M) Volume of Oxygen evolved(ml) Average Concentration of Oxygen Evolved (ml) 1.00 65.0 65.0 63.0 64.3 0.80 53.0 51.0 51.0 52.0 0.60 38.0 38.0 39.0 38.3 0.40 28.0 28.0 27.0 28.0 0.20 15.0 14.0 14.0 14.3 0.00 3.00 4.00 3.00 3.30 Calculating the Rate the Reaction. Rate (ml/s) =Volume of oxygen released (ml) Time(s) TABLE SHOWING THE RATE OF REACTION AT DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE Concentration Of Hydrogen Peroxide(M) Average Concentration of Oxygen Evolved Time (s) Rate (ml) 1.00 64.3 37.2 1.73 0.80 52.0 40.0 1.23 0.60 38.3 42.4 0.90 0.40 28.0 49.3 0.57 0.20 14.3 51.0 0.28 0.00 3.30 59.5 0.55 From the results, I have noticed that the volume of the products (Oxygen) ...read more.


Because in this experiment I used a constant enzyme concentration, decrease in substrate concentration results in less or limited number of enzyme- substrate complexes as the enzyme's active sites are not working to full capacity, this is because there is less chance of enzymes colliding with the substrate molecules. Which is why there is decrease in the volume of the products and the rate of reaction in the 0.00M concentration of Hydrogen Peroxide? Substrate concentration is limiting the rate of this reaction. However, if the substrate concentration was increased to lets say; 2M, I think the graph of "Average Volume Against Hydrogen Concentration" would tail off when it reaches the maximum point called the" V-max ".The reaction would then become constant because the active sites of the enzymes would gradually become fully utilised and adding more substrate would have no effect on the rate of the reaction. ...read more.


So it has % Error = 1 X 100=1% 100 2. Difficulties in reading the highest volume reached by the froth. The meniscus of the froth was not clear. 3. Inadequate washing of the measuring cylinder-some Hydrogen Peroxide may have remained in the measuring cylinder The above errors can be improved by:- 1. Replacing the measuring cylinder with a more accurate apparatus e.g. Burette. 2. Using the method of displacement to collect the Oxygen gas. It has a good and more accurate way of taking the volume of the oxygen because there is no froth. 3. Use of different measuring cylinder for each dilution, this will lessen the errors because there would be no need to rewash the same measuring cylinder. I don't think the test is reliable because there is too many minor errors which have a great effect on the results. I suggest the use of a manometer would be more reliable and would generally improve the test. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The effect of aspirin on the action of bovine liver catalase

    each other with one overlap between the minimum value for 15gdm-3 of aspirin and the maximum value for 20gdm-3 of aspirin (1.65mm3s-1 of oxygen produced). However, because there is a clear overall reduction and no overlaps in the rest of the data, it may be assumed that the data is

  2. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    and 16 & 20 vols. is very small. These were the stages in the reaction where the enzymes had begun to limit the rate of reaction. The substrate concentration in 12, 16 and 20 vols. was extremely high and each time, limitation in the reaction's progress was similar.


    Conical flask Used to mix all the reactions in. It is large enough to contain all the reactants together and also the most suitable as the probe can fit inside monitoring the pH. Beaker Used to contain boiling water, allowing me to create my own water bath, also used as a store for sodium carbonate and cream.

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Increase in the rate of reaction as the hydrogen peroxide increases. Water A high volume of water slows the reaction between the hydrogen peroxide and the catalase. This is because; the dilution reduces the number of catalase present. Therefore; the number of active sites are limited.

  1. Investigation into the effect of substrate concentration on the rate of hydrogen peroxide decomposition ...

    the rate of reaction will remain constant even though more enzymes are present. In this experiment, this variable controlled by adding the same amount of potato for each reaction. The potatoes are cut into cylinders of identical diameter and length.

  2. The effect of concentration on the activity of catalase.

    When two molecules react, bonds are broken or made and there often is a rearrangement of the atoms. According to the collision theory, for this to happen, the molecules must collide first. An effective collision must occur. An effective collision is the number of collisions that result in a reaction.

  1. Effect Of Substrate Concentration On The Activity Of Catalase

    The table below shows what amounts of Hydrogen Peroxide and water are needed to make the solutions. Concentration Of Hydrogen Peroxide Volume Of Hydrogen Peroxide (cm3) Volume Of Water (cm3) 20% 4 0 16% 3.2 0.8 12% 2.4 1.6 10% 2 2 8% 1.6 2.4 4% 0.8 3.2 0% 0 4 5.

  2. To examine the effect of Substrate Concentration (Hydrogen Peroxide) on the rate of an ...

    and it will enable us to answer our brief as it will show the effect of changing concentration against rate of reaction. We will obtain the gradients for the original (o2 evolution/time) graphs by drawing a line against the curve of the graph where it begins to level off.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work