• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of HydrogenPeroxide Concentration On Catalase

Extracts from this document...

Introduction

INVESTIGATING THE EFFECT OF HYDROGEN PEROXIDE CONCENTRATION ON CATALASE ANALYSIS TABLE SHOWING THE AMOUNT OF OXYGEN EVOLVED AT THE DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE. Concentration of Hydrogen Peroxide(M) Volume of Oxygen evolved(ml) Average Concentration of Oxygen Evolved (ml) 1.00 65.0 65.0 63.0 64.3 0.80 53.0 51.0 51.0 52.0 0.60 38.0 38.0 39.0 38.3 0.40 28.0 28.0 27.0 28.0 0.20 15.0 14.0 14.0 14.3 0.00 3.00 4.00 3.00 3.30 Calculating the Rate the Reaction. Rate (ml/s) =Volume of oxygen released (ml) Time(s) TABLE SHOWING THE RATE OF REACTION AT DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE Concentration Of Hydrogen Peroxide(M) Average Concentration of Oxygen Evolved Time (s) Rate (ml) 1.00 64.3 37.2 1.73 0.80 52.0 40.0 1.23 0.60 38.3 42.4 0.90 0.40 28.0 49.3 0.57 0.20 14.3 51.0 0.28 0.00 3.30 59.5 0.55 From the results, I have noticed that the volume of the products (Oxygen) ...read more.

Middle

Because in this experiment I used a constant enzyme concentration, decrease in substrate concentration results in less or limited number of enzyme- substrate complexes as the enzyme's active sites are not working to full capacity, this is because there is less chance of enzymes colliding with the substrate molecules. Which is why there is decrease in the volume of the products and the rate of reaction in the 0.00M concentration of Hydrogen Peroxide? Substrate concentration is limiting the rate of this reaction. However, if the substrate concentration was increased to lets say; 2M, I think the graph of "Average Volume Against Hydrogen Concentration" would tail off when it reaches the maximum point called the" V-max ".The reaction would then become constant because the active sites of the enzymes would gradually become fully utilised and adding more substrate would have no effect on the rate of the reaction. ...read more.

Conclusion

So it has % Error = 1 X 100=1% 100 2. Difficulties in reading the highest volume reached by the froth. The meniscus of the froth was not clear. 3. Inadequate washing of the measuring cylinder-some Hydrogen Peroxide may have remained in the measuring cylinder The above errors can be improved by:- 1. Replacing the measuring cylinder with a more accurate apparatus e.g. Burette. 2. Using the method of displacement to collect the Oxygen gas. It has a good and more accurate way of taking the volume of the oxygen because there is no froth. 3. Use of different measuring cylinder for each dilution, this will lessen the errors because there would be no need to rewash the same measuring cylinder. I don't think the test is reliable because there is too many minor errors which have a great effect on the results. I suggest the use of a manometer would be more reliable and would generally improve the test. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The effect of aspirin on the action of bovine liver catalase

    However, because the overall reduction between an aspirin concentration 0gdm-3 and 20 gdm-3 is large compared to the standard deviations, with an overall reduction of nearly 1mm3s-1 or percentage reduction of 37%, it is quite safe to suggest tentatively that aspirin inhibits the action of bovine liver catalase.

  2. Effect Of Substrate Concentration On The Activity Of Catalase

    Arefin Khan Aim: Investigate the effect of temperature on the activity of catalase (from potato). Introduction: Enzymes are biological catalysts. They speed up metabolic reactions in the body but remain chemically unchanged themselves. Enzymes contain an active site. This is a region, normally a depression or cleft, to which another molecule may bind.

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    This can simply be done by shaking the beakers or flasks gently which contains the solutions ensuring nothing spills. 3. Measure out 10ml of full fat cream using a pipette and add into a beaker labelled cream. You must ensure that you wash the pipette as it will be required once measuring other substances.

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Another factor kept as a constant variable is the time limit allowed for the oxygen to be produced. As to keep the results detailed so that quick changes can be identified; a low time limit is more effective. For fair testing as well as for the control of the outcome of the experiment, temperature must also be considered.

  1. The effect of concentration on the activity of catalase.

    When two molecules react, bonds are broken or made and there often is a rearrangement of the atoms. According to the collision theory, for this to happen, the molecules must collide first. An effective collision must occur. An effective collision is the number of collisions that result in a reaction.

  2. Study the reaction of Catalase and Hydrogen Peroxide. I am specifically focusing on the ...

    This will show how much of the catalase is reacting with the substrate. Prediction I predict that the higher the substrate concentration the higher the amount of Oxygen produced in the time given or the higher the rate of reaction.

  1. To examine the effect of Substrate Concentration (Hydrogen Peroxide) on the rate of an ...

    and it will enable us to answer our brief as it will show the effect of changing concentration against rate of reaction. We will obtain the gradients for the original (o2 evolution/time) graphs by drawing a line against the curve of the graph where it begins to level off.

  2. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    The larger the surface area of a substance, the faster the rate of reaction. This is because there are more particles per cm3. Therefore, there will be more collisions every second between the substrate molecules and the enzymes. Volume is another key factor that can influences the rate of the reaction between hydrogen peroxide and catalyse.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work