• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of HydrogenPeroxide Concentration On Catalase

Extracts from this document...


INVESTIGATING THE EFFECT OF HYDROGEN PEROXIDE CONCENTRATION ON CATALASE ANALYSIS TABLE SHOWING THE AMOUNT OF OXYGEN EVOLVED AT THE DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE. Concentration of Hydrogen Peroxide(M) Volume of Oxygen evolved(ml) Average Concentration of Oxygen Evolved (ml) 1.00 65.0 65.0 63.0 64.3 0.80 53.0 51.0 51.0 52.0 0.60 38.0 38.0 39.0 38.3 0.40 28.0 28.0 27.0 28.0 0.20 15.0 14.0 14.0 14.3 0.00 3.00 4.00 3.00 3.30 Calculating the Rate the Reaction. Rate (ml/s) =Volume of oxygen released (ml) Time(s) TABLE SHOWING THE RATE OF REACTION AT DIFFERENT CONCENTRATION OF HYDROGEN PEROXIDE Concentration Of Hydrogen Peroxide(M) Average Concentration of Oxygen Evolved Time (s) Rate (ml) 1.00 64.3 37.2 1.73 0.80 52.0 40.0 1.23 0.60 38.3 42.4 0.90 0.40 28.0 49.3 0.57 0.20 14.3 51.0 0.28 0.00 3.30 59.5 0.55 From the results, I have noticed that the volume of the products (Oxygen) ...read more.


Because in this experiment I used a constant enzyme concentration, decrease in substrate concentration results in less or limited number of enzyme- substrate complexes as the enzyme's active sites are not working to full capacity, this is because there is less chance of enzymes colliding with the substrate molecules. Which is why there is decrease in the volume of the products and the rate of reaction in the 0.00M concentration of Hydrogen Peroxide? Substrate concentration is limiting the rate of this reaction. However, if the substrate concentration was increased to lets say; 2M, I think the graph of "Average Volume Against Hydrogen Concentration" would tail off when it reaches the maximum point called the" V-max ".The reaction would then become constant because the active sites of the enzymes would gradually become fully utilised and adding more substrate would have no effect on the rate of the reaction. ...read more.


So it has % Error = 1 X 100=1% 100 2. Difficulties in reading the highest volume reached by the froth. The meniscus of the froth was not clear. 3. Inadequate washing of the measuring cylinder-some Hydrogen Peroxide may have remained in the measuring cylinder The above errors can be improved by:- 1. Replacing the measuring cylinder with a more accurate apparatus e.g. Burette. 2. Using the method of displacement to collect the Oxygen gas. It has a good and more accurate way of taking the volume of the oxygen because there is no froth. 3. Use of different measuring cylinder for each dilution, this will lessen the errors because there would be no need to rewash the same measuring cylinder. I don't think the test is reliable because there is too many minor errors which have a great effect on the results. I suggest the use of a manometer would be more reliable and would generally improve the test. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The effect of aspirin on the action of bovine liver catalase

    in rate of reaction is -0.10, the percentage difference is -6%, so a variability of � 4.6% in the results for 20gdm-3 means that these results cannot be used to prove that there is a difference in rate of reaction between aspirin concentrations of 15gdm-3 and 20gdm-3.


    pipette has been washed out so that the sodium carbonate does not react with anything before the experiment begins, this will result in accurate and precise results. 8. Measure out 10ml of lipase into test tube labelled lipase using pipette again make sure it has been rinsed out of any chemicals.

  1. Effect Of Substrate Concentration On The Activity Of Catalase

    As the scale on the pipettes shows the volume to the nearest mm3 the volume of the solutions that I used should be correct to the nearest mm3. The volume of gas in the test tube to start with is slightly affected by the amount which the bung is pushed

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    However; if a really high concentration of substrates were added to the enzyme concentration, the reaction would stay constant. This is until the enzymes have broken down the other substrates; the ones that have not been broke down would "wait" until the enzymes are free for them.

  1. The effect of concentration on the activity of catalase.

    Figure 1 illustrates the action of increasing the concentration. The reaction of the decomposition of the hydrogen peroxide by catalase is a redox reaction. This experiment uses catalase as the enzyme. Catalase is an enzyme that is present in the cells of plants, animals and aerobic bacteria.

  2. To examine the effect of Substrate Concentration (Hydrogen Peroxide) on the rate of an ...

    will enable us to achieve our aim of finding out the effect of changing substrate concentration on the rate of an enzyme controlled reaction. Any anomalous results will be repeated to check that they are correct, Ideally the whole experiment would be repeated 3-5 times so that an average set

  1. Investigate the effect of changing substrate concentration on the rate of the reaction between ...

    The larger the surface area of a substance, the faster the rate of reaction. This is because there are more particles per cm3. Therefore, there will be more collisions every second between the substrate molecules and the enzymes. Volume is another key factor that can influences the rate of the reaction between hydrogen peroxide and catalyse.

  2. Study the reaction of Catalase and Hydrogen Peroxide. I am specifically focusing on the ...

    This means it can be reactivated if the pH rises or falls again. However, if the pH rises or falls to the extremes the enzyme may be denatured, meaning it cannot be resurrected. Input and Output Variables In my experiment I am going to investigate the affect changing the concentration

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work