• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Chromatography of photosynthetic pigments. Aim: To determine the different photosynthetic pigments found in spinach and find their relative front values using paper chromatography.

Extracts from this document...

Introduction

Chromatography of Photosynthetic Pigments Aim: To determine the different photosynthetic pigments found in spinach and find their relative front values using paper chromatography. By grinding the spinach leaves, its pigments were extracted in order to run a thin layer of chromatography film. The relative front values for every photosynthetic pigment are found by dividing 'a' (distance each pigment travelled from the loading line in cm) by 'b' (distance solvent travelled from the same loading line in cm). Pigment Color Carotene Yellow Phaeophytin Yellow-grey Xanthophyll Yellow-brown Chlorophyll a Blue-green Chlorophyll b green Calculations for the Relative Fronts of Different Pigments Pigment a (cm) ...read more.

Middle

This is because carotene is more water soluble than chlorophyll a and is able to move up the chromatography paper with the solvent through capillary actions until the dipole-dipole forces between carotene and the solvent weakens and breaks. This process leaves a mark at a certain height on the paper. Chromatogram is the pattern pigments form as they are revealed as color streaks on the chromatography paper. All pigments absorb different wavelengths of light and this explains the different rates at which the pigments climbed up the chromatography paper. ...read more.

Conclusion

This is why plants tend to appear to be green to our eyes. In this lab, chlorophyll a showed the greatest difference between standard and experimental Rf values: 0.15 Overall, the standard and experimental Rf values were fairly close to each other for all the pigments detected in the spinach leaves. Errors * Bending or changing the position of the filter paper in the cylinder during the paper chromatography process. Result: difficulty distinguishing the individual pigment bands. * Not grinding the spinach leaves well Result: harder to distinguish the different pigments present from each other. Conclusion By going through the process of paper chromatography, various pigments present in the spinach leaves were made visible to our eyes: carotene, phaeophytin, canthophyll, chlorophyll a, and chlorophyll b. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Chromatography lab. Title: separation of pigments of photosynthesis using paper chromatography.

    Data collection and processing: Points of each color appearance 1st trial Solvent front=6 2nd trial Solvent front=6 d Rf D Rf X1 2.5 0.42 3.5 0.58 X2 4.8 0.80 4.0 0.67 X3 4.9 0.82 5.8 0.960.02 Table(1): experiment's raw data * (d): distance that the pigment moved.

  2. Plant Cromatography Lab Report. How does the use of different colored leaves affect the ...

    * 4 beakers (100 ml � 0.5 ml) Procedure The data collected throughout the experiment will be displayed in a data table. The experiment can be repeated twice, so the data table will represent results for both trials and the average.

  1. Biodiversity Investigation The aim of this investigation is to determine plant biodiversity of both ...

    from counting plant species and quantity in Location 1 Location 1 Region A (Quadrats next to the pond) Region B (Quadrats 10 feet away) Rye Grass 6 Dandelions Legumes 3 Rumex 8 Chinese Fire Weed Mint 15 Clovers 14 Bermuda Grass 18 Figure 1.2 - Raw Data obtained from counting

  2. Title: separation of pigments of photosynthesis using paper chromatography. Goal (main aim): ...

    - Compare results with the standard Rf table.(This helps to determine the type of pigment obtained). Data collection and processing: Points of each color appearance 1st trial Solvent front=6 2nd trial Solvent front=6 d Rf D Rf X1 2.5 0.42 3.5 0.58 X2 4.8 0.80 4.0 0.67 X3 4.9 0.82

  1. Biological rhythms. In order to say that such a clock exists, it is essential ...

    Organisms, including plants and humans, continue their rhythms when placed in the deepest mine shafts or when orbited in satellites but all research thus far indicates that the oscillator for circadian rhythms is endogenous (internal) . This internal clock, however, is entrained (set)to a period of precisely 24 hours by

  2. Photosyntetic Pigments

    The wavelength of light determines its color. For example, The wavelength of red is about 700 nm and the wavelength of blue light is about 470 nm. Graph.1: Made by Raffaela Pastore Meneguetti Visible light is a part of a larger spectrum of radiation called the electromagnetic spectrum.

  1. Biology - Observing the Process of Guttation

    lower transpiration rate; therefore, they will perform the process of guttation by exuding beads of water through its hydathodes. On the other hand, I predict that the green bean sprouts grown in the exposed containers will not go through the process of guttation as they will experience lower humidity and a higher transpiration rate.

  2. Chromatography of Photosynthetic Pigments

    The rate at which the pigments travel up the chromatography paper is dependent on the solubility of the substance in the solvent used and the adherence of the substance to the paper. The photosynthetic pigments evident after the lab were not so obvious by observing the green spinach as a whole.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work