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Lab Enzymes Activity
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The effect of different pH value
on the activity of enzymes
Introduction:
Amylase is an enzyme which catalyses hydrolytic digestion of polysaccharides. In human's serum 8 isoenzymes of alpha-amylase can be detected. They all come from pancreas, salivary glands, mucous membrane, mammary gland, ovaries and testes.
Contact of food with mucous membrane of oral cavity and even the very view or smell of food causes secretion of saliva. Saliva is produced in the amount of about 0,5 liter per 24 hours and is of pH 7,0. Solid foods in oral cavity are fragmented and mixed with saliva. Saliva contains enzyme digesting polysaccharides called 'salivary alpha - amylase (salivary amylase)'.
The salivary amylase causes initial digestion of foods in the digestive system.
Aim:
The aim of this investigation is learning how enzyme amylase's activity and speed of digestion is influenced by different pH levels.
Hypothesis:
As amylase is secreted in saliva and pH of saliva is 7,0 pH we assume that this value of pH is an optimal value for the activity of this enzyme. The amylase therefore will best digest starch when pH
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