• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Use of Recombinant DNA Technology

Extracts from this document...


The Use of Recombinant DNA Technology Recombinant DNA technology is the idea, which has been realized as well, to cut DNA with the help of restriction enzymes from two different sources and then join bits of the DNA with the help of DNA ligase back together, to get a use out of this recombined DNA. Recombinant DNA technology is closely linked to Genetic Modification. As there are three ways to use genetically modification, recombinant DNA technology deals with the idea to insert a foreign gene into a DNA of a host. The other two methods are to either alter an existing and active gene in the host DNA or to completely switch it of. The idea behind introducing different DNA into an already existing DNA is to get from the bacteria or organism a different effect. As certain sequences of the DNA, which are called genes code for certain proteins regulate the protein production of the host DNA. To realize this from the theory into real life the theory needs the use of recombinant DNA technology. Everything starts with the DNA and its sequences. As scientists have studied what certain proteins do, they followed the way back they came from. A protein is formed by amino acids, which mRNA is coding for. ...read more.


As now a plasmid is taken and is cut with the same restriction enzyme then the DNA sequence is going to have the same sticky ends. To be able to recognize later on the plasmid, which has been prepared the plasmid carriers two genes inside itself. These genes inside the plasmid make the bacteria resistant against the antibiotics ampicillin and tetracycline. A recognition sequence lies within the tetracycline gene, where the restriction enzyme made a cut. The cut plasmid and sequence of DNA are put together. Naturally the complementary sticky ends (as mentioned before same restriction enzyme) are attracted to each other and then bonded by adding the enzyme DNA ligase. The recombinant plasmid is introduced into bacterial cells, by just adding the DNA to the bacterial, which picks it up through the terms of transformation. From this point on cloning of the ``new�� plasmid starts as the bacteria is reproducing. To actually now be able to recognize the recombinant plasmid in the bacteria from the other bacteria, because not all of the bacteria picked the plasmid up a copy is taken from the bacteria cultures. The original culture is now tested on tetracycline. As mentioned above the tetracycline resistant gene has been taken apart by restriction enzyme at our new plasmid, because a recognition side lied within it. ...read more.


into the zeprafish genome. The protein is a natural produced green bioluminescence from a jellyfish. The fish is as well able to fertilize. At first the experiment was for developing a fish detecting pollution, but already in 2003 the trademarked protected fish called ``GloFish�� went on the Taiwan market for $18.60 each. In December 2003 the fish was allowed to be sold in the US as well, as the firm of GloFish made various experiments and there was and is apparently no danger from a fish, which contains recombinant DNA (statement of the Glofish firm). Until now the fish is not allowed in Europe, because it is containing recombinant DNA or it is genetically modified. Overall Recombinant DNA Technology is a great way to discover a lot of things, because there is just so much to actually experiment with. My opinion is that the development of this technology should not stop. Scientist should be allowed to research for things that could be beneficial to the human kind, but everything has to be controlled and serious. No messing around with genes, like I think the GloFish is. Sources http://learn.genetics.utah.edu/units/basics/print-and-go/gel_electro.cfm http://academic.brooklyn.cuny.edu/biology/bio4fv/page/genetic-engin/restriction%20enzyme.html http://www.urdugame.com/the-glofish/ "Troubled waters: fluorescent fish spark GM row" by A. Gumbel, May 4th, The Independent, London, England ``Biology for the IB DIPLOMA�� Chris Clegg 2007, fist published in 2007 by Hodder Murray ``Senior Biology 1�� 2008 Student Workbook, Richard Allan 2007, published by BIOZONE International Ltd Word count: 1407 ?? ?? ?? ?? Lucas Schumacher 26/02/2008 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Research Question: Which method of DNA extraction (using a centrifuge or not) will yield ...

    This supported my hypothesis that a centrifuge would yield more DNA. The papaya yielded more DNA than the banana and the strawberry. This supports my hypothesis that the papaya would yield the most DNA. This is due to polyploidy-as the most genetically advanced fruit, the papaya has the most number of chromosomes.

  2. Browning Enzyme

    This graph has been added as to the standard deviation and the error bars of data that excludes its anomalies. The length of all the errors bars are quite long and so this shows by excluding the anomalies A high standard deviation exists which indicates a broad range of possible values relative to the expected value.

  1. Biology- Extended essay. For this research, I investigated the effects of DDT and ...

    85 83 79 82 2.4494897 0.06 A 76 80 84 84 84 81.6 3.5777088 B 71 78 86 70 88 78.6 8.2945765 C 75 72 79 84 80 78 79.88 4.6368093 5.11 Array 2 D 84 76 78 80 74 78.4 3.8470768 E 76 82 88 88 80 82.8 5.2153619

  2. A Brief summary of the Life of Rosalind Franklin. Rosalind Franklin, ...

    In 1947, Rosalind traveled to Paris and spent roughly three to four years at the labratoire Central des Services Chimques de I'Etat. An associate by the name of Adrienne Weill helped her to get a position at that facility (Msn Encarta).

  1. IB Genetic Unit Notes

    Dominant allele - an allele that has the same effect on the phenotype whether it is present in the homozygous or heterozygous state. Recessive allele - an allele that only has an effect on the phenotype when present in the homozygous state.

  2. Should Animals have the same rights as Humans? Both animals and humans exhibit behaviours ...

    In addition, the data could be seen as invalid because the dogs that participated were strays, their breed or background was unknown. Knowing their background would enable one to determine what sort of conditioned reflexes have already been established in relation to a given stimulus and to theorise on how this might affect the results.

  1. Biotechnology Assignment: Genetic Screening/Testing

    This type of result is called uninformative, indeterminate, inconclusive, or ambiguous. Uninformative test results sometimes occur because everyone has common, natural variations in their DNA called polymorphisms, which do not affect health. If a genetic test finds a change in DNA that has not been associated with a disorder in

  2. Biology: Enzymes in the Dairy Industry

    Technologically, lactose crystallizes easily which sets limits to certain processes in the dairy industry, and the use of lactase to overcome this problem has not reached its fullest potential because of the associated high costs.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work