Methods
One experiment was performed to determine the effects of salinity on the establishment Pearl Barley seed germination individually. Firstly, three treatments ware prepared for seed to produce different salinity environment. For this step, 58 grams of salt were dissolved in 1 liter container to made standard salt solution. Then, it can be calculated by formula C1V1=C2V2 to find out quantities of standard salt solution and pure water to mixed, in order to get specific concentration of a solution, which is 0 millimolar, 300 millimolar and 600 millimolar. Secondly, soak the seed by fully cover 50 seeds in 3 different concentration of solution for 24 hours in the dark respectivelly. Following, germination dishes were prepared by putting wet blotter paper and labeling for three containers. Then, 40 presoaked seeds from 3 treatment groups were added to the matched container and replaced lid. The seeds started to germinate after period of time, the record the number of seeds germinate in each container everyday.
Results
The number of seeds that germinated in different treatment group was all decreasing by the increasing salinity (Figures 1, 2 and 3). Moreover, the number of fenugreek seeds germination is more than number of barley seeds germination by different concentration, as 20 fenugreek seeds germinate at 600 millimolar NaCl solutions (Figure 2), but no barley seed germinate at 600 millimolar NaCl solutions (Figure 3). Also, all the most number of seeds germinated in the control solution which is 0 millimolar NaCl solutions(Figure 1, Figure 2 and Figure 3).
A significant increase of germination frequencies in the presence of NaCl was observed in seeds
from salt-treated plants, as compared to seeds from control, non-treated plants.
Figure
Personal data:
Figure 1: Individual result of Barley (Hordeum vulgare) seeds germination by different NaCl concentration
Class data:
Figure 2: Class result of Fenugreek (Trigonella foenum-graecum) seeds germination by different NaCl concentration
Figure 3: Class result of Barley (Hordeum vulgare) seeds germination by different NaCl concentration
Discussion
The personal data from individual experiment is similar to the class data. For example, the number of barley seeds germination by 0 millimolar and 300 millimolar from personal data is 2 seeds more than class data, and for 600 millimolar both have same result which is no seed germinated. It may be states that individual experiment was established in a correct way, and environment for the seeds was pretty average condition. Refer to graphs, germination of fenugreek seeds are much more than barley seeds between class data, likely, there are 20 seeds germinated in highest salt concentration treatment, but no barley seed germinated in this treatment. It can be concludes that these two plants have different levels of salt tolerance.
Reference
(Villagra, P.E. and Cavagnaro, J.B. 2005)
Villagra, P.E. and Cavagnaro, J.B. (2005) Effects of salinity on the establishment and early
growth of Prosopis argentina and Prosopis alpataco seedlings in two contrasting soils:
Implications for their ecological success. Austral Ecology 30; 325-335.
(Boscaiu, M. et cl. 2005)
Boscaiu, M., Estrelles, E., Soriano P. and Vicente, O. (2005) Effects of salt stress on the
reproductive biology of the halophyte Plantago crassifolia. Biologia Plantarum 49: 141-143.
(Branson R.L. and Gustafson C.D. 1971)
Branson R.L. and Gustafson C.D. (1971) Irrigation Water-A Major Salt Contributor To Avocado Orchards, California Avocado Society 1971-72 Yearbook 55: 56-60
Australian Bureau of Statistics (2003) ‘Environmental impacts of agriculture’, in Australian Bureau of Statistics website, accessed 13 September 2009, From: <http://www.abs.gov.au/Ausstats/[email protected]/Previousproducts/1301.0Feature%20Article162003?opendocument&tabname=Summary&prodno=1301.0&issue=2003&num=&view=>
