• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

nasal carriage of s.aureus

Extracts from this document...

Introduction

´╗┐Analytical microbiology lab report Name : Apu sarwar Class : analytical and pharmaceutical chemistry Date of practical: 2/10/12 and 9/10/12 and 16/10/12 ________________ Title: Estimation of the prevalence of nasal carriage of Staphylococcus aureus in the nasal passages of this class of college students.(three part all together) Objective: This prospective investigation on healthy college student is undertaking to estimate the prevalence of S.aureus nasal carriage and identify the micro organism by using different media and test like gram stain. Introduction: Staphylococci are often found in the human nasal cavity (and on other mucous membranes) as well as on the skin. S. aureus is with some frequency found as normal human flora, Approximately 30% of adults and most children are healthy periodic nasopharyngeal carriers of S. aureus. Staphylococcus aureus (coagulate-positive staphylococci). Staphylococcus aureus is the most pathogenic species and is implicated in a variety of infections. to diagnostic them it is very important to identify this microorganism. there is many way the micro organism can identify. Here different media and staining will apply to identify the organism. ...read more.

Middle

The left and right anterior nares were swabbed four times around. The swabs were immediately inoculated in to two different medium Mannitol salt agar (MSA) and in a Nutrient agar. The Mannitol salt agar (MSA) was incubated at 37°C (7 days); the Nutrient agar culture plates were also incubated at 37°C (7 days). Identification of S. aureus was based upon colony morphology and gram stain. Gram stain was performed for the nasal sample during the 1st class for this practical. A mix result was found in the microscope from the stain. There was some pink and purple bacteria ware present. which means the gram positive and gram negative coocoi was present in the sample because gram negative bacteria lost their cell wall by stain and get pink in colour and gram positive stay purple. the result was mixed because there other bacteria could have been present in the nose sample or it could have been contaminated from air.but in the experiment the target bacteria is S.aureus. ...read more.

Conclusion

to identify the pathogen bacteria, the agar was flooded with 1N HCl for 10 min and then the agar was analysed again there was a clear zone around the colony. which indicated that the pathogenic staphylococci was present. Because at the present of HCl the DNA is polymerised and medium gets opaque, and the organism produce DNAse enzyme and hydrolysis the DNA and make clear zoon around the colony. In the sample there was Staphylococcus aureus because the clear zone was in large area which indicate that it was Staphylococcus aureus not Staphylococcus epidermidis because epidermidis produce very small clear zoon. There was 34 student was given the sample from there nose and 74 % was found S aureus positive. which was higher then expect value. The reason could be the some human error during the experiment also there could have some problem with identification of the clear zoon in the DNAse agar. Conclusion: This study provides a representative assessment of carriage of S aureus in the college student.The results suggest that nearly one in four of the population is currently not colonized by S aureus. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our University Degree Microbiology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related University Degree Microbiology essays

  1. Use of selective and differential media to identify bacteria

    Nutrient agar is firstly inoculated as all of the four bacterium are able to grow in that media as compared to the MacConkey media 1 and 3. MacConkey 1 and 3 is then inoculated as it contains additives such as dyes which could inhibit the growth of certain bacterium when exposed to it.

  2. Free essay

    Identifying microorganisms using differential staining techniques

    heating, and staining The chemical that is responsible for the acid-fast property of Mycobacteria is mycolic acids. Due to the high levels of mycolic acids present in the cell walls of Mycobacteria, ordinary stains are not sufficient in penetrating the walls.

  1. Bacterial Metabolism and Enzymatic Growth

    It was present in P. aeruginosa, as indicated by the cloudiness surrounding the colony, however was no present in Bs or Ec. The results of Bs did not concur with the expected results. Bs was expected to produce lipase, but was not observed in the experimental results.

  2. Antigen-Antibody Interactions: an analysis

    This leads to decrease in the inter-particle space and subsequently increases the number of collisions, removing the lag period. Hawkins 1964 reports that the optical properties exhibited by precipitates formed in the various regions of the precipitin curve are not identical.

  1. Distinguishing Species of Bacteria

    (Madigan and Thomas, 2009) Pseudomonas aeruginosa is a common Gram- negative, aerobic, rod-shaped bacterium which can cause disease in animals. (Madigan and Thomas, 2009) Bacillus subtilis is a Gram-positive, catalase-positive bacterium commonly found in soil. Proteus vulgaris is a rod-shaped, Gram negative bacterium that inhabits the intestinal tracts of humans and animals.

  2. This review looks at how silicification of bacteria allows preservation of the bacteria through ...

    van den Boorn SHJM, van BergenMJ, Nijman W, Vroon PZ (2007) Dual role of seawater and hydrothermal fluids in Early Archean chert formation: Evidence from silicon isotopes. Geology 35, 939-942. 6. Hofmann A, Bolhar R (2007) Carbonaceous Cherts in the Barberton Greenstone Belt and Their Significance for the Study of Early Life in the Archaean Record.

  1. A review of positive and negative impacts of microbes on the environment

    ethanol, by the degradative activities of microorganisms', (Madigan, et al, 2003: 8). It is some of these microbes that have been isolated for pollution prevention and site remediation which has lead to a process known as bioremediation. 'When bioremediation is applicable, it is often a cost-effective means of restoring environmental quality', (Atlas and Bartha, 1998: 556).

  2. The objective of this lab was to determine if different cultures would successfully grow ...

    It contains tryptone (digest of casein), soytone (digest of soybeans), sodium chloride, agar and water (Dickson, 2002). The reason why a good majority of bacteria can grow on TSA is because casein, and soytone provide amino acids to the bacterial culture (Dickson, 2002).

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work