Introduction:
During summer last year, I had a rash, more like pimples only on my right cheek. I thought it was because of the heat. Although this was the first time I ever had such kind of skin problem, I didn’t pay much attention. An aunt told me that it could be because of the pillow and not the heat. To my surprise, after basking my pillowcase in the sun for a day, the rash or pimples started to disappear! It is common sense that exposing clothes to direct sunlight not only makes the clothes smell fresh, but also disinfects. I started to believe that bacteria or fungi caused the pimples or rash on my right cheek as I sleep on my right side only.
Hypothesis:
My hypothesis is that micro organisms inhabit the part of the pillow used more often and infects the skin. Exposing the suspected materials to sunlight may prevent this.
Methods:
Cut a piece of the pillow (unwashed for a week); cut this piece into two equal parts (2 cm2). Cut the same size from a washed and clean pillowcase (2 nos.). Expose 1 unwashed piece and 1 washed piece to sunlight for 10 hours and keep the other 2 pieces inside a house. Collect all four samples after 10 hours and inoculate directly to an already prepared sterilized agar media in a petridish. Incubate at room temperature for 3 days and observe for any bacterial growth. Count the bacterial growth under sterilized condition and record data. Keep it for another 4 days (that makes it 7 days) and repeat the observation for fungal growth.
Expected results:
I would expect the data to be like the following
Table 1: Number of bacterial or fungal count in each sample.
* cfu = Colony Forming Unit
Conclusion:
If I obtain the above data approximately, I would conclude that my hypothesis, that the rash or pimples is caused by micro organisms, is true. I would then suggest that exposing clothes to direct sunlight once in a while, especially those which are generally used away from sunlight, is a good practice to avoid unnecessary skin infection.
If the microbial growth count in the unwashed piece left unattended is equivalent to the growth count in the unwashed piece exposed to sunlight and the washed piece left unattended, then I would conclude that the rash or pimples on the skin is not necessarily caused by micro organisms and would reject my hypothesis.