Part B-Making a Wet Mount
- Obtained a clean microscope slide and cover slip.
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Cut out a 3mm2 sample from the colored comics section of a newspaper and used the medicine dropper to place a drop of water on the slide.
- Placed the comics sample on the slide and added an additional drop.
- Placed the cover slip on the slide at a 45° angle.
- Viewed the slide under all three powers starting with the low-power objective lens.
- Recorded observations.
- Repeated steps 3-4 using a sample of newsprint from the classified section. Placed the slide so the words were easily read.
- Viewed the slide under the lower-power objective lens.
- Recorded observations.
- Removed the slide.
- Cleaned up the lab area and placed the equipment in the designated places.
Observations
Summary of Results
Description of Color Comics
Low power: Blurry, dark outlines, string of pink, yellow, and green
Medium power: dark outlines, string of yellow, green and orange, pink blotch on the side
High power: detailed string of yellow and green, dark outlines, pink blotch on the side
Diagram of Classified Ads Under Low Power
Part A- Using the Microscope
- The image appears more detailed, focused and sharper in the medium magnification than in the low magnification.
- The slide appears to move to the left when it is moved to the right.
- The slide appears to move to the right when it is moved to the left.
- The image appears larger, more detailed, and focused in the high magnification than in the medium magnification.
- The diaphragm adjusts the amount of light of the microscope.
Calculations
Magnification Power X Magnification Power = Total Magnification
of Ocular Lens of Objective Lens
- 4 X 10 = 40
- 10 X 10 = 100
- 40 X 10 = 400
Conclusions
The purpose was achieved. Since the enlargement of images under certain magnifications using the lenses and light of a microscope was seen, it can therefore be concluded that a microscope enables one to see details that are otherwise too small to be seen with the unaided eye by the use of lenses and light to magnify an object. As a result, my hypothesis was proven correct.
Text Questions
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a) Objective Lenses: lenses used to focus when using the microscope
b) Stage clips: clips on top of the stage used to hold slides
c) Course-adjustment knob: round knob on the side of the microscope used for focusing on the specimen
d) Fine-adjustment knob: round knob on the side of the microscope used to fine-tune the focus of the specimen after using the coarse adjustment knob
e) Revolving nosepiece: the rotating part of the microscope at the bottom of the body tube that holds the objective lenses
f) Diaphragm: controls and adjusts the amount of light
- One should watch the objective lens from the side when changing magnification because it allows the individual to prevent the lens from scratching the slide.
- The higher the magnification, the less the specimen is seen.
- The higher the magnification, the more detail that is visible.
- One would have to move the slide up and to the right to move an image from the top-right to the center of the field of view.
- One does not see the minute details of the comic, like the strips of colors, without the aid of a microscope.
- The letters of the newsprint seem to be backwards and have higher detail under the microscope than with the unaided eye.
- A microscope should be stored covered when not in use to prevent dust from accumulating.