Report comparing different growth media, aseptic techniques and laboratory safety with own experiences

Authors Avatar by zeynep_merve10hotmailcouk (student)

Part 1-

As the practical work had been carried out in a Category 1 microbiology laboratory, the required aseptic technique was to abide by personal hygiene rules such as washing hands before and after carrying out the three experiments, avoiding eating and drinking within the laboratory and tying the hair back. Personal Protective Equipment (PPE) that is required, consists of a lab coat; gloves and eye protection is worn if necessary. Also, benches are wiped before and after handling specimen in order to make a cleaner environment. The wastes which have had contact with the specimen are autoclaved before further disposal, this is done to prevent infectious microbes from being released into the environment.

In Category 2 laboratories, microbiologists deal with pathogenic and/or infectious microorganisms that tend to have a moderate hazard. Due to their potential to cause diseases in humans, they are dealt with great care to prevent injuries that can be through needle sticks and cuts. Contact with broken glassware is prohibited and decontamination of sharp material before disposal is strictly required.

Therefore, category 2 laboratories are required to provide special safety facilities and equipment such as the laminar flow cabinet which provides a sterile - particle-free- working environment with the help of air projecting through a system of filtration and carrying it across a working surface in a laminar air stream. There are three classes in biosafety cabinets, class I & II can be used in group 1,2 & 3 Microbiology Laboratories; whereas a class III cabinet will be used in a group 4 Microbiology Laboratory.

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Class I biosafety cabinet provide protection for the user by allowing the air to flow inwards. This way the dirt particles that form within the cabinet escape through filtration. However, the work inside the cabinet is not protected from contamination.

 Class II biosafety cabinets have the potential of protecting the user, product and the environment through a system that controls the contamination effect of airborne particles and reduces the possibility of the user from being exposed to them.

Class III cabinets provide a totally enclosed working area. The safety cabinet is continuously supplied with filtered air and the exhaust air is drawn ...

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