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Investigate the effect of changing the substrate concentration on the rate of reaction of hydrogen peroxide in the presence of catalase enzyme

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Investigate the effect of changing the substrate concentration on the rate of reaction of hydrogen peroxide in the presence of catalase enzyme Aim The aim of this experiment is to prove that the concentration of substrate does have an impact on the speed of a reaction. If there is enough substrate the enzyme will continuously have substrates in its active site which it will be turning into products. The speed of the reaction will be dependent on the speed of the enzyme. However if there is less substrate available the reaction will be slower because enzymes will not have as many substrate to turn into to product Prediction I predict that less time will be taken for the bead to reach the surface of the Hydrogen Peroxide when the Hydrogen Peroxide is less diluted. This is because, when the enzyme and the substrate are first mixed together there is a large number of substrate molecules and each enzyme has a substrate attached to its active site at any one moment. The speed of the reaction is dependent on the speed at which the enzyme can turn the substrate in its active site into a product and attach to another substrate so the enzyme is working at its maximum speed. ...read more.


I will draw up 5ml of the mixture used to make beads for each bead to make them the same size. Apparatus 100ml Calcium Chloride 1.4% 100ml of 0.05g Catalase 10ml 0f 2% Sodium Alginate 5ml Glycerol 1 litre 20 volume Hydrogen Peroxide 5 Test tubes 5 drops Borax Carmine 100ml Distilled Water for dilution Stopwatch Chinagraph Pencil Goggles Glass rods Beaker for mixing Spatula Measuring Cylinders (10ml) Teat Pipettes A small Beaker 5 test tube rack 1 water bath set at 50??? I have chosen to use a stopwatch because it's a lot more reliable than looking at a clock or watch as it gives you the semi seconds as well and also it always you to focus on the bead moving from the surface to the bottom and back, unlike with a watch or clock were you would have to look at the time and not the bead. The Chinagraph Pencil is so that you do not confuse the test tubes and get inaccurate results. Measuring cylinders are needed to measure accurate quantities of the above liquids. Method 1) Place a mixture of sodium alginate and glycerol in a small beaker. ...read more.


20) As soon as it touches the surface again stop the stopwatch. 21) Repeat 3 times and record results. 22) Use the closest two results to work out the average time taken. This will make your results more accurate. 23) To work out the average for the closest two results you use the following equation: result 1 + result 2 � 2 = average result 24) Repeat the steps 15 to 23 for the remaining test tubes. Risk Assessment 1) Hydrogen Peroxide is considered to be a mutagen and so must be handled with care. 2) Do not touch it with your hands 3) It's a corrosive chemical and can cause irritation to eyes and skin. 4) Goggles must be worn at all times. 5) Breathing hydrogen peroxide can cause irritation to the nose and throat. 6) Avoid inhaling it at all times. 7) Further breathing of it cause irritation to the lungs and result in coughing and shortness of breath 8) Do not deliberately inhale substance 9) Further exposure can result in a build up of fluid in the lungs which would require immediate medical attention 10) Do not eat any bead or drink any liquids 11) Wash hands thoroughly after experiment. 12) Wipe any spills with a paper towel. 19/02/07 Audrey Chikasha ...read more.

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