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DNA Strawberry Extraction Lap

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Introduction

Lab : DNA Strawberry Extraction Hypothesis Strawberries have DNA that has the ability to be extracted and also to be seen under a microscope. Explanation of Hypothesis All livings things have some sort of genetic code. Strawberries are not an exception to this rule, and in strawberries this genetic code is called DNA. The DNA has the ability to be extracted due to it having a different molecular nature than the rest of the cell. Variables Dependent The amount of DNA extracted from the strawberry Independent The size of the strawberries Fixed Time of which ethanol and strawberries are left together Temperature of ice in which the ethanol is held Amount of extraction buffer used Amount of ethanol used Type of strawberries used Type of microscope used Time of which strawberries are smashed for Time of which extraction buffer and strawberries are mixed together Strainer used Uncontrolled Microscope visibility Room temperature External light Observation Watch carefully and record what you observe where the ethanol meets the filtrate layer. The solution separates into two layers, with the red filtrate at the bottom, the white ethanol on top, with some air bubble like mixture of both in the middle. Let the solution sit for two minutes without disturbing it. Describe the precipitate. A clump of pinkish air bubbly DNA with countless tiny bubbles form from the filtrate at the bottom. ...read more.

Middle

essential elements as advanced laboratory DNA extraction procedures, such as, mechanical disruption of cells, liberation of the DNA, and precipitation of the DNA. 6) Which human cells would you use for DNA extraction and why? The most common human cells that would be used for DNA extraction are hair, skin and saliva because there is an abundant source of them and it is painless and easy to obtain it. Blood is also easy to obtain, but a bit of pain can be side effect. 7) Why do you think scientists study the DNA of strawberries? Scientists use the study of DNA because strawberries have enormous genomes, they are octoploid (8 copies of each gene). They also produce pectinases and cellulases which start the breakdown of cell walls as they ripen. 8) Is the DNA pure? What other molecule normally associated with DNA will be present? No, DNA is not pure. It also has protein molecules embedded within it. For example, there are strawberry proteins because it is slightly pink. 9) Some people are concerned that manipulation of DNA will change life as we know it. Provide two examples of human genes that might be modified. Human genes have been modified to provide resistance to illnesses and diseases, especially genetic diseases. ...read more.

Conclusion

Evaluations Throughout the whole lab, one can find possible errors (weaknesses and limitations) - Did not specify a specific temperature for the cold ethanol. May cause erroneous outcomes in the separation of DNA and the solution. - Getting the DNA off the inoculating loop was problematic. It is possible that the DNA was mashed and affected during this procedure of applying the DNA onto a slide. Hand was needed. - May cause erroneous results in the efficiency of the buffer due to a specific type of shampoo for the mixing of the DNA extraction buffer. - No specific safety rules or conduct was implied or listed. May cause danger or injury to experimenters. In order to improve this DNA Strawberry Extraction for further usage, the following improvements can be made in respect to the weaknesses and limitations found above. - Provide and state another way of getting the DNA off the inoculating loop. Possibly, the use of another inoculating loop, the use of toothpicks, or even the use of hands. - Specify, in the procedures or materials, a specific temperature for the cold ethanol and one should be able to maintain that temperature.. - Specify, in the materials, a specific type of shampoo and strawberry for the mixing of the DNA extraction buffer. - List or state some safety rules or conduct regarding the lab. Even small safety details never hurts. ?? ?? ?? ?? Ryan Cho Block A Apr/ 2/ 08 ...read more.

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