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Enzyme Experiment Design

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Introduction

Enzyme Activity Introduction Enzymes are globular protein, which is made up by chains of amino acids, that acts as a catalyst for biochemical reaction. Enzyme will be able to work as a catalyst when a substrate (a reactant in a biochemical reaction) is present and bind with the active site on enzyme, which is the area on the surface of an enzyme. In this experiment, the enzyme is catalase and the substrate is hydrogen peroxide. Using catalase hydrogen peroxide will break down into water and oxygen, which can be shown by the chemical equation: 2H2O2 2H2O + O2 Also, the rate of enzyme activity can be affected by different factors including temperature, pH level and substrate concentration. Aim In this experiment, I will be investigating how the change in hydrogen peroxide concentration can affect the rate of enzyme activity. In order to do this I will measure the amount of oxygen produced in a certain period for different concentration of hydrogen peroxide. ...read more.

Middle

By keeping the temperature constant can prevent it acting as a contributing factor that will affect the reliability of my results. â»Throughout the experiment, I will only be using 1% catalase solution. â»The volume of catalase (3ml) and hydrogen peroxide(2ml) is to be keep constant 3ml catalase, by using measuring cylinders. â»Time – I will only measure the amount of oxygen produced in the first 10 seconds of the reaction. 1. Uncontrolled Variable â»pH level of hydrogen peroxide concentration: Concentration of H2O2 (%) 0 10 20 30 40 50 60 70 80 90 100 pH of H2O2 at 25â 7.0 5.3 4.9 4.7 4.6 4.5 4.5 4.5 4.6 4.9 6.2 source: http://h2o2.com/faqs/FaqDetail.aspx?fId=26 As shown from the table above, different concentration of hydrogen peroxide has different pH level. It can’t be possible for me to keep the pH level of hydrogen peroxide constant while changing its concentration; therefore even though pH level may affect the rate of enzyme activity, I cannot control the pH level as the change in pH level may also change the concentration of hydrogen peroxide. ...read more.

Conclusion

2. Prepare 15ml of 1% catalase and 10ml of 20% hydrogen peroxide. 3. Use measuring cylinder and pipette to get 3ml of 1% catalase and 2ml of 20% hydrogen peroxide. Check the measurements at eye level to ensure that the measurements are exact. And pour each solution into a test tube. Repeat this for 5 times. 4. Pour the catalase into the conical flask. Pour a test tube that contains 2ml of 20% hydrogen peroxide into the conical flask. 5. Have the stopwatch ready and quickly cover the conical flask with the stopper that is attached to the delivery tube and immediately at timing with the stopwatch 6. When the time almost reaches to 10 seconds keep your eyes close to the plunger for the amount of oxygen produced. Pull the stopper out from the flask and record the result down. 7. Push the plunger back and rinse conical flask 8. Repeat steps 2 to 9 for five times for each different hydrogen peroxide concentration. (Rinse beaker once you change the concentration of hydrogen peroxide) ...read more.

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