• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzyme IA Biology

Extracts from this document...


Enzyme Lab Aim: Investigate the effect of a factor that influences enzyme activity Background: Scientists attended a Pre Lab discussion about enzymes reviewing the many factors that should commonly affect enzymes such as temperature, poison, pH, number of enzymes and solubility. The role of enzymes was also reviewed in order for students to develop their own lab. Enzymes are proteins which serve as a catalysts, a chemical agent that changes the rate of a reaction without being consumed by the reaction. Every reaction before starting has a hill of energy that it must first get over before it can begin its reaction known as the Energy of Activation. This energy of activation is much higher without the enzyme present and with the enzyme is lowered to the point where reactants can turn into products more readily. In regards to temperature, higher temperatures speed up all reactions but at a certain temperature enzymes begin to become denatured(deactivated) and at even higher temperatures 60 � or more they only continue to become less effective. Research Question: At what temperature does the catalase of lived produce the most Oxygen in a reaction of hydrogen peroxide? Hypothesis: If the catalase's temperature is changed by both heating and cooling then the catalase will be most effective in terms of amount of Oxygen produced (ml) ...read more.


Have the other Partner 1 hold stopper w/ tube and the Pipet filled with 1ml of Liver solution e. Have partner 2 place tube inside 100ml graduated cylinder i. With graduated cylinder underwater lift about 2 inches ii. Push tube inside cylinder iii. Slowly lower 100ml graduated cylinder down until tube Is secured in cylinder f. Have partner 1 note starting time by looking at the clock g. In the same second squirt Pipet filled with 1ml of Liver solution into test tube with 10ml of Hydrogen Peroxide h. As quickly as possible stop the test tube with the stopper i. Have partner 1 count out 20 seconds by looking at the clock j. After 20seconds record amount of amount of oxygen produced in graduated cylinder. (this will be the amount of water that escaped outside of the cylinder noted by the empty space in the graduated cylinder) 11. Repeat step (10) 4 more times 12. Cool Liver solution to 0�C a. Pour 10ml of Liver solution into 10ml graduated cylinder b. Remove bottom piece of graduated cylinder leaving only the cylinder left c. Place thermometer inside cylinder d. Dig small space in ice tub with enough space for 10ml cylinder e. ...read more.


However while it is slightly off balance it is still a viable conclusion supported by outside sources in form of the pre-lab discussion regarding the tendencies of an enzyme to perform "In regards to temperature, higher temperatures speed up all reactions but at a certain temperature enzymes begin to become denatured (deactivated) and at even higher temperatures 60 � or more they only continue to become less effective." In spite of the fact that this conclusion is supported by pre-lab discussion there is still room for improvement should this lab be conducted again. First in order to assure the accuracy of liver/water ratio the procedure should be modified in step 5 d to add an additional step to scrape out liver stuck to weightboat so that the proper liver/water ratio can be certain. Second the addition of an additional partner should be introduced into the procedure so that the test tube can be stopped more effectively by a third partner allowing the second partner to focus on holding the test tube and the first partner to focus on emptying the pipet. This in turn will make up for whatever oxygen was lost to the lab environment and instead allow that lost oxygen to be recorded in the data. Incrament ?? ?? ?? ?? Towstik 7 Wesley Towstik Towstik 1 Biology 2B 10/10/10 Enzyme Lab ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    Effect of substrate concentration on catalase activity (Biology IA)

    3 star(s)

    Conclusions As seen by the varying times taken for the coloured liquid to rise by 5cm (seconds) ?0.01seconds at different concentration of hydrogen peroxide, all pieces of chicken liver have differing reactions to the hydrogen peroxide due to the different amounts of catalase present in the tissue.

  2. Biology HL IA - Transpiration lab report

    number of stomata, plant may take water at the same rate even after leaves are cut. This could happen as a result of continuous transpiration pull. Maybe if the measurements would have been taken the following day after all the leaves were cut they would be similar as expected, e.g.

  1. Browning Enzyme

    The discs were of the same diameter as I has used the corker borer, however * there was the uncertainty of the ruler and so the thickness for all the discs were not the same and this was evident

  2. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    Increasing the Hydrogen Peroxide Concentration after the point of saturation has been reached will not cause the rate of reaction to go up any more. All the active sites are being used so any extra Hydrogen Peroxide molecules will have to wait until an active site becomes available.

  1. IB biology Respiration IA

    and simultaneously start the timer. Record the time in seconds that it takes for the BTB to start to change color. Discard the BTB in the sink and rinse out the test tube thoroughly. 5. Repeat steps 1-4 2 more times, increasing the exercise jumping jacks by 30 seconds each time.

  2. The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in ...

    The mean relative reaction rate is about 0.24/s for 40�C and about 0.08/s for 60�C, both of which are smaller than the mean relative reaction rates of 0�C and 20�C (0.28/s and 0.29/s respectively).When it reaches 80�C there is no reaction occurring meaning a mean relative reaction rate of 0/s.

  1. How does changing the temperature of green beans affect the activity of the peroxidase ...

    at our highest rate of 0.35 mL s-1 recorded at 30 degrees. The curve of best fit is reasonably accurate and it passes through all of the error bars. This indicates that the trend indicated by the line is accurate.

  2. Biology: Enzymes in the Dairy Industry

    Cheese ripening is a complex process mediated by biochemical and biophysical changes during which a bland curd is developed into a mature cheese with characteristic flavour, texture, and aroma. The desirable attributes are produced by the partial and gradual breakdown of carbohydrates, lipids, and proteins during ripening, mediated by several agents (i)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work