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Enzyme IA Biology

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Introduction

Enzyme Lab Aim: Investigate the effect of a factor that influences enzyme activity Background: Scientists attended a Pre Lab discussion about enzymes reviewing the many factors that should commonly affect enzymes such as temperature, poison, pH, number of enzymes and solubility. The role of enzymes was also reviewed in order for students to develop their own lab. Enzymes are proteins which serve as a catalysts, a chemical agent that changes the rate of a reaction without being consumed by the reaction. Every reaction before starting has a hill of energy that it must first get over before it can begin its reaction known as the Energy of Activation. This energy of activation is much higher without the enzyme present and with the enzyme is lowered to the point where reactants can turn into products more readily. In regards to temperature, higher temperatures speed up all reactions but at a certain temperature enzymes begin to become denatured(deactivated) and at even higher temperatures 60 � or more they only continue to become less effective. Research Question: At what temperature does the catalase of lived produce the most Oxygen in a reaction of hydrogen peroxide? Hypothesis: If the catalase's temperature is changed by both heating and cooling then the catalase will be most effective in terms of amount of Oxygen produced (ml) ...read more.

Middle

Have the other Partner 1 hold stopper w/ tube and the Pipet filled with 1ml of Liver solution e. Have partner 2 place tube inside 100ml graduated cylinder i. With graduated cylinder underwater lift about 2 inches ii. Push tube inside cylinder iii. Slowly lower 100ml graduated cylinder down until tube Is secured in cylinder f. Have partner 1 note starting time by looking at the clock g. In the same second squirt Pipet filled with 1ml of Liver solution into test tube with 10ml of Hydrogen Peroxide h. As quickly as possible stop the test tube with the stopper i. Have partner 1 count out 20 seconds by looking at the clock j. After 20seconds record amount of amount of oxygen produced in graduated cylinder. (this will be the amount of water that escaped outside of the cylinder noted by the empty space in the graduated cylinder) 11. Repeat step (10) 4 more times 12. Cool Liver solution to 0�C a. Pour 10ml of Liver solution into 10ml graduated cylinder b. Remove bottom piece of graduated cylinder leaving only the cylinder left c. Place thermometer inside cylinder d. Dig small space in ice tub with enough space for 10ml cylinder e. ...read more.

Conclusion

However while it is slightly off balance it is still a viable conclusion supported by outside sources in form of the pre-lab discussion regarding the tendencies of an enzyme to perform "In regards to temperature, higher temperatures speed up all reactions but at a certain temperature enzymes begin to become denatured (deactivated) and at even higher temperatures 60 � or more they only continue to become less effective." In spite of the fact that this conclusion is supported by pre-lab discussion there is still room for improvement should this lab be conducted again. First in order to assure the accuracy of liver/water ratio the procedure should be modified in step 5 d to add an additional step to scrape out liver stuck to weightboat so that the proper liver/water ratio can be certain. Second the addition of an additional partner should be introduced into the procedure so that the test tube can be stopped more effectively by a third partner allowing the second partner to focus on holding the test tube and the first partner to focus on emptying the pipet. This in turn will make up for whatever oxygen was lost to the lab environment and instead allow that lost oxygen to be recorded in the data. Incrament ?? ?? ?? ?? Towstik 7 Wesley Towstik Towstik 1 Biology 2B 10/10/10 Enzyme Lab ...read more.

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