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Experiment - The amount of cell division in the Allium cepa root tip cell

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Introduction

The amount of cell division in the Allium cepa root tip cell Mitosis is the main part of cell division. It is a process where a single cell divides resulting in two identical cells, each containing the same number of chromosomes and genetic content as that of the original cell. It consists from four phases respectively: * Prophase o Formation of chromosomes o Disappearance of the nuclear envelope * Metaphase o Chromosomes align along the equator of the cells * Anaphase o Chromosomes are split into two sister chromatids by spindle fibers and are pulled to the poles of the cell * Telophase o Two new nuclei are formed and the cytoplasm undergoes division This experiment will show us how many of the cells undergo division in an area of the root tip. ...read more.

Middle

Count all of the cells with a visible nucleus 4. Identify and count the cells that undergo a certain phase of mitosis Phase Example Prophase Metaphase Anaphase Telophase 5. Estimate the number of cells that undergo interphase a. All cells without the cells undergoing mitosis 6. Pick an area above the previous area and repeat steps 3-5 a. The area should be exactly above the area before Data # of cells (individual data data) # of cells (group average) Phases View #1 View #2 Interphase 461 351 Prophase 8 11 Metaphase 4 2 Anaphase 3 6 Telophase 2 1 Total 478 371 Phases View #1 avg View #2 avg Interphase 263.3 182.6 Prophase 51.4 25.86 Metaphase 7.7 5.3 Anaphase 5.6 4.7 Telophase 3.1 1.9 Total 294.7 195.2 The data presented is individual data and the group's average. ...read more.

Conclusion

Counting each and every cell is slow o Another mechanism would be counting the number of rows, counting the amount of cells in 1/5 of them, multiply by 5 Conclusion Cells with clear nucleuses were observed. Cells were arranged in rows; most of them had a rectangular shape, some were close to a shape of a square. There was a big amount of the ones in interphase, and small amounts of the ones in mitosis. Cells were in different phases of mitosis or in interphase and were distinguished clearly. Even though the data through the group was various, the expectations were proven correct. The order of cells in different phases was in this: interphase, prophase, metaphase, anaphase and telophase. These results were caused by the different amount of time needed for each phase. ...read more.

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